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Zymographic Analysis and Characterization of MMP-2 and -9 Forms in Human Sound Dentin
Inhibition of MMP-2 and -9 proteolytic activity may slow caries progression and increase the durability of resin-dentin bonds. Expand
Evidence for redifferentiation of human chondrocytes grown on a hyaluronan-based biomaterial (HYAff 11): molecular, immunohistochemical and ultrastructural analysis.
The data indicate that human chondrocytes seeded on HYAFF 11 express and produce collagen type II and aggrecan and downregulate the production of collagen type I, providing an in vitro demonstration for the therapeutic potential of HYAff 11 as a delivery vehicle in a tissue-engineered approach towards the repair of articular cartilage defects. Expand
Immunocytochemical detection of phosphatidylinositol 4,5-bisphosphate localization sites within the nucleus.
It is shown that a significant amount of the phospholipid is retained by the cytoskeleton and by the inner nuclear matrix in in situ matrix preparations and the sensitivity of the immunocytochemical reaction is capable of detecting quantitative variations of PIP2 nuclear content induced by agonists that modulate the signal transduction system at the nuclear level. Expand
Push-out stress for fibre posts luted using different adhesive strategies.
Within the limitations of the study, the use of an etch-and-rinse adhesive in combination with a dual-cure cement to lute fiber posts is the most stable luting procedure if compared with a self-etch resin-based cement or aSelf-adhesive cement, as assayed by thermocycling of the bonded specimens. Expand
MMP-2 assay within the hybrid layer created by a two-step etch-and-rinse adhesive: biochemical and immunohistochemical analysis.
The results of this study support the use of non-toxic MMPs inhibitors, such as CHX, as an appropriate additional step in bonding procedures in order to increase the longevity of the adhesive restorations. Expand
High-resolution detection of newly synthesized DNA by anti-bromodeoxyuridine antibodies identifies specific chromatin domains.
We analyzed the incorporation of bromodeoxyuridine (BrdUrd) into DNA in exponentially growing murine erythroleukemia cells (FLC-745), using fluorescent anti-BrdUrd antibodies with light microscopyExpand
Analysis of differential artificial ageing of the adhesive interface produced by a two-step etch-and-rinse adhesive.
NaOCl solution is a rapid and reliable in vitro ageing method for examining the durability of the adhesive interface produced by two-step etch-and-rinse adhesive systems. Expand
Differential kinetics of propidium iodide uptake in apoptotic and necrotic thymocytes
The kinetics of propidium iodide incorporation in vivo in isolated rat thymocytes can be used instead of DNA staining of ethanol-treated cells or nick translation to recognize apoptotic cells, and distinguish apoptosis from necrosis, without killing the cell. Expand
Nuclear morphology during the S phase
The results suggest that DNA replication occurs at a nucleosomal‐like fiber level and that replicating enzymes machinery moves over a fixed template. Expand
Nanoleakage within the hybrid layer: a correlative FEISEM/TEM investigation.
  • P. Suppa, L. Breschi, +6 authors F. Tay
  • Materials Science, Medicine
  • Journal of biomedical materials research. Part B…
  • 1 April 2005
The results of the present study confirm the hypothesis that both total- and self-etch adhesives are not able to fully infiltrate the dentin substrate. Expand