Structural Basis for Gluten Intolerance in Celiac Sprue
A 33-mer peptide was identified that has several characteristics suggesting it is the primary initiator of the inflammatory response to gluten in Celiac Sprue patients, and could be detoxified in in vitro and in vivo assays by exposure to a bacterial prolyl endopeptidase, suggesting a strategy for oral peptidase supplement therapy for CeliacSprue.
Comparative biochemical analysis of three bacterial prolyl endopeptidases: implications for coeliac sprue.
The present work has illustrated the diverse nature of this class of enzymes from the standpoint of proteolysing complex substrates such as gluten, and demonstrated the relative utility of alternative prolyl endopeptidases for treating coeliac sprue.
Intestinal digestive resistance of immunodominant gliadin peptides.
- F. Hausch, L. Shan, N. Santiago, G. M. Gray, C. Khosla
- BiologyAmerican Journal of Physiology - Gastrointestinal…
- 1 October 2002
It is shown that these proline-glutamine-rich epitopes from alpha-gliadin are exceptionally resistant to enzymatic processing, and a possible enzyme therapy strategy for celiac sprue is suggested.
Identification and analysis of multivalent proteolytically resistant peptides from gluten: implications for celiac sprue.
The pivotal role of physiologically generated, proteolytically stable, TG2-reactive, multivalent peptides in the immune response to dietary gluten in Celiac Sprue patients is highlighted.
Starch digestion and absorption in nonruminants.
- G. M. Gray
- Chemistry, MedicineJournal of NutriLife
By virtue of this sequential luminal and membrane digestion followed by glucose transport, starch is assimilated in a very efficient manner in nonruminants.
Different populations of pig epidermal cells: isolation and lipid composition.
The relative amounts of glycosphingolipid (glucosylceramide) and cholesteryl sulfate in the total lipids of stratum corneum cells were less than half those in the granular cells and basal and spinous cells.
Intestinal surface peptide hydrolases: identification and characterization of three enzymes from rat brush border.
Lipid compositions of cells isolated from pig, human, and rat epidermis.
Epidermal slices from pig, human, and rat skin were treated with dilute buffered trypsin solution (0.005%, w/v), and suspensions of mixed basal and spinous cells were obtained in good yield. Total…
Localization and composition of lipids in neonatal mouse stratum granulosum and stratum corneum.
- P. Elias, B. Brown, P. Fritsch, J. Goerke, G. M. Gray, R. White
- BiologyJournal of Investigative Dermatology
- 1 November 1979
It is suggested that lamellar bodies secrete glycolipids and free sterols, which are then extensively metabolized to free fatty acids, ceramides, and sterol esters, which coalesce to form the broad bilayers of the stratum corneum interstices.
Glycosphingolipids and ceramides in human and pig epidermis.
The glycosphingolipids and ceramides in the stratum corneum which contain high proportions of long chain saturated and 2-hydroxy fatty acids with high melting points appear well-suited to withstand wide changes in temperature, ultraviolet radiation and atmospheric oxidation that may occur within the environment of the skin surface.