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Direct transesterification of all classes of lipids in a one-step reaction.
This one-step direct transesterification procedure carried out in methanol-benzene 4:1 with acetyl chloride is superior to currently used methods not onlyBecause of its simplicity and speed, but also because of its added precision.
Improved recovery of fatty acid through direct transesterification without prior extraction or purification.
The direct transesterification method appears to be particularly advantageous for the recovery of the highly volatile medium chain triglycerides and there is no need to add an antioxidant to protect unsaturated lipids.
The potential of the hydrocarbon breath test as a measure of lipid peroxidation.
Specific methylation of plasma nonesterified fatty acids in a one-step reaction.
The specificity of the methylation reaction for NEFA without hydrolysis of other classes of plasma lipids was substantiated with appropriate standards and this one-step specific methylation procedure is superior to currently used methods.
Direct transesterification of plasma fatty acids for the diagnosis of essential fatty acid deficiency in cystic fibrosis.
- G. Lepage, E. Levy, N. Ronco, L. Smith, N. Galeano, C. Roy
- MedicineJournal of lipid research
- 1 October 1989
This study aimed at redefining criteria for essential fatty acid (EFA) deficiency with the use of the direct transesterification procedure and determining whether a simple assay of total fatty acids (FA) is as predictive of EFA deficiency as the FA pattern from plasma, red cell, and platelet phospholipids.
Circulating lipids and lipoproteins in glycogen storage disease type I with nocturnal intragastric feeding.
- E. Levy, L. Thibault, C. Roy, M. Bendayan, G. Lepage, J. Letarte
- Biology, MedicineJournal of lipid research
- 1 February 1988
The data suggest that glycogen storage disease type I patients on nocturnal intragastric feeding remain at risk for atherosclerosis and its complications.
Contiguous deletion of the X-linked adrenoleukodystrophy gene (ABCD1) and DXS1357E: a novel neonatal phenotype similar to peroxisomal biogenesis disorders.
Three newborn boys who had clinical symptoms and initial biochemical results consistent with PBD or SED are identified, and it is shown that they lacked ALDP, and deletions that extended into the promoter region of ABCD1 and the neighboring gene, DXS1357E are identified.
Increased levels of plasma malondialdehyde in Friedreich ataxia.
Several lines of evidence support the hypothesis that similar mechanisms are involved in the human disease, and data from yeast suggest that frataxin deficiency results in mitochondrial dysfunction and free radical damage.
Effect of an organized lipid matrix on lipid absorption and clinical outcomes in patients with cystic fibrosis.
OLM is a readily absorbable source of fat and energy in CF and is an effective nutritional supplement that led to better clinical outcomes in terms of energy intake from the diet, weight-for-age Z score, essential fatty acid status, vitamin E, and retinol binding protein.
Hepatobiliary disease in cystic fibrosis: a survey of current issues and concepts.
The possibility that correction of the abnormalities of bile acid metabolism could slow the progress or prevent CF cirrhosis is discussed in light of recent experimental data.