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Structural basis for LFA-1 inhibition upon lovastatin binding to the CD11a I-domain.
TLDR
L lovastatin, a drug clinically used for lowering cholesterol levels, inhibits the interaction of human LFA-1 with its counter-receptor intercellular adhesion molecule-1, and the first three-dimensional structure of an integrin inhibitor bound to its receptor is revealed.
NMR solution structure of the inserted domain of human leukocyte function associated antigen-1.
TLDR
The solution structure of the Mg(2+) complex of the I-domain of LFA-1 has been determined by NMR methods, using a model-based approach to nuclear Overhauser enhancement spectroscopy peak assignment and the role of divalent cations appears not to be as a direct mediator of a conformational change that alters affinity for the ligand.
Biophysical characterization of DNA aptamer interactions with vascular endothelial growth factor
TLDR
The approach effectively maps the secondary structural elements in the free aptamer, which present the unpaired interface for high affinity VEGF recognition, and demonstrates that a directed binding analysis can be used in concert with library screening to characterize and improve aptamer/ligand recognition.
SANE (Structure Assisted NOE Evaluation): An automated model-based approach for NOE assignment
TLDR
A semi-automated procedure for complete NOESY assignment (SANE, Structure Assisted NOE Evaluation), coupled to an iterative procedure for NMR structure determination where the user is directly involved.
ZZ domain of CBP: an unusual zinc finger fold in a protein interaction module.
Solution structure of the cysteine-rich domain of the Escherichia coli chaperone protein DnaJ.
TLDR
Zinc (15)N-(1)H HSQC titrations indicate that the fold of the isolated DnaJ CR domain is zinc-dependent and that one zinc module folds before the other, and this sequence conservation is likely to result in a conserved structural motif.
The structure of a tunicate C-type lectin from Polyandrocarpa misakiensis complexed with D -galactose.
TLDR
Analytical ultracentrifugation revealed that TC14 behaves as a dimer in solution, and this is reflected by the presence of two molecules in the asymmetric unit with the dimeric interface formed by antiparallel pairing of the two N-terminal beta-strands and hydrophobic interactions.
Novel MicroRNA Candidates and miRNA-mRNA Pairs in Embryonic Stem (ES) Cells
TLDR
Perturbation of these pairs in the ES (GCNF −/−) mutant suggests a role for miRNAs in the core regulatory networks underlying ES cell self-renewal, pluripotence and differentiation, and a plethora of previously unknown miRNA candidates were revealed.
LFA-1 Affinity Regulation Is Necessary for the Activation and Proliferation of Naive T Cells*
TLDR
It is found that blocking high affinity LFA-1 prevents interleukin-2 production and T cell proliferation, demonstrated by TCR cross-linking and antigen-specific stimulation.
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