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Natural Competence in the Hyperthermophilic Archaeon Pyrococcus furiosus Facilitates Genetic Manipulation: Construction of Markerless Deletions of Genes Encoding the Two Cytoplasmic Hydrogenases
ABSTRACT In attempts to develop a method of introducing DNA into Pyrococcus furiosus, we discovered a variant within the wild-type population that is naturally and efficiently competent for DNAExpand
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SurR: a transcriptional activator and repressor controlling hydrogen and elemental sulphur metabolism in Pyrococcus furiosus
This work describes the identification and characterization of SurR, Pyrococcus furiosus sulphur (S0) response regulator. SurR was captured from cell extract using promoter DNA of a hydrogenaseExpand
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Deletion strains reveal metabolic roles for key elemental sulfur-responsive proteins in Pyrococcus furiosus.
Transcriptional and enzymatic analyses of Pyrococcus furiosus previously indicated that three proteins play key roles in the metabolism of elemental sulfur (S(0)): a membrane-bound oxidoreductaseExpand
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Exploiting microbial hyperthermophilicity to produce an industrial chemical, using hydrogen and carbon dioxide
Microorganisms can be engineered to produce useful products, including chemicals and fuels from sugars derived from renewable feedstocks, such as plant biomass. An alternative method is to use lowExpand
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Mechanistic insights into energy conservation by flavin-based electron bifurcation.
The recently realized biochemical phenomenon of energy conservation through electron bifurcation provides biology with an elegant means to maximize utilization of metabolic energy. The mechanism ofExpand
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Single gene insertion drives bioalcohol production by a thermophilic archaeon
Significance The microbial production of ethanol (bioethanol) is a massive commercialized technology. Though alcohols with longer carbon chains are chemically much better suited for currentExpand
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Recombinogenic Properties of Pyrococcus furiosus Strain COM1 Enable Rapid Selection of Targeted Mutants
ABSTRACT We recently reported the isolation of a mutant of Pyrococcus furiosus, COM1, that is naturally and efficiently competent for DNA uptake. While we do not know the exact nature of thisExpand
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Bioprocessing analysis of Pyrococcus furiosus strains engineered for CO2‐based 3‐hydroxypropionate production
Metabolically engineered strains of the hyperthermophile Pyrococcus furiosus (Topt 95–100°C), designed to produce 3‐hydroxypropionate (3HP) from maltose and CO2 using enzymes from the MetallosphaeraExpand
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Mutational Analyses of the Enzymes Involved in the Metabolism of Hydrogen by the Hyperthermophilic Archaeon Pyrococcus furiosus
Pyrococcus furiosus grows optimally near 100°C by fermenting carbohydrates to produce hydrogen (H2) or, if elemental sulfur (S0) is present, hydrogen sulfide instead. It contains two cytoplasmicExpand
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A New Class of Tungsten-Containing Oxidoreductase in Caldicellulosiruptor, a Genus of Plant Biomass-Degrading Thermophilic Bacteria
ABSTRACT Caldicellulosiruptor bescii grows optimally at 78°C and is able to decompose high concentrations of lignocellulosic plant biomass without the need for thermochemical pretreatment. C. besciiExpand
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