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Organization of vesicular trafficking in epithelia
TLDR
Recent live-cell-imaging studies provide a real-time view of sorting processes in epithelial cells, including key roles for actin, microtubules and motors in the organization of post-Golgi trafficking.
An Annexin 2 Phosphorylation Switch Mediates p11-dependent Translocation of Annexin 2 to the Cell Surface*
TLDR
It is shown that endothelial cell annexin 2, a protein that lacks a typical signal peptide, translocates from the cy toplasm to the extracytoplasmic plasma membrane in response to brief temperature stress both in vitro and in vivo in the absence of cell death or cell lysis.
Kinesin and dynamin are required for post-Golgi transport of a plasma-membrane protein
TLDR
Experiments test the hypothesis that when the characteristic central localization of the Golgi is preserved, microtubules, kinesin and the GTPase dynamin are essential for post-Golgi trafficking and show that kinesIn and Dynamin are required for different stages of post-golgi transport.
cdc42 regulates the exit of apical and basolateral proteins from the trans‐Golgi network
TLDR
It is reported here that mutants of cdc42 inhibited the exit of basolateral proteins from the trans‐Golgi network (TGN), while stimulating theexit of an apical marker, in two different transport assays, suggesting regulation may result from modulation of the actin cytoskeleton, as GTPase‐deficient cDC42 depleted a perinuclear actin pool that rapidly exchanges with exogenous fluorescent actin.
KIF17 stabilizes microtubules and contributes to epithelial morphogenesis by acting at MT plus ends with EB1 and APC
TLDR
This paper presents a large number of novel mechanisms leading to cell reprograming in Nocodazole-naïve mice, and investigates the role of these mechanisms in the development of Huntington’s disease.
Three-dimensional analysis of post-Golgi carrier exocytosis in epithelial cells
TLDR
Quantitative analysis suggested that basolateral, but not apical, PGTIs fused with the lateral membrane in polarized cells, correlating with the restricted localization of Syntaxins 4 and 3 to lateral and apical membrane domains, respectively.
Dynein- and microtubule-mediated translocation of adenovirus serotype 5 occurs after endosomal lysis.
TLDR
Fluorescence ratio imaging revealed a neutral pH in the environment of translocating Ad, leading to a model in which the interaction of Ad with an intact microtubule cytoskeleton and functional cytoplasmic dynein occurs after escape from endosomes and is a necessary prerequisite to nuclear localization of adenovirus serotype 5.
Detyrosination of tubulin regulates the interaction of intermediate filaments with microtubules in vivo via a kinesin-dependent mechanism.
TLDR
Tubulin detyrosination functions as a signal for the recruitment of IFs to MTs via a mechanism that is likely to involve kinesin, and is found to be inhibited by the same reagents that disrupted the IF-Glu MT interaction in vivo.
Leukocyte transmigration requires kinesin-mediated microtubule-dependent membrane trafficking from the lateral border recycling compartment
TLDR
It is reported that targeted recycling from this lateral border recycling compartment (LBRC) is required for diapedesis, is mediated by kinesin family molecular motors, and requires normally functioning endothelial microtubules.
LIM kinase 1 and cofilin regulate actin filament population required for dynamin-dependent apical carrier fission from the trans-Golgi network.
TLDR
It is suggested that TGN-localized LIMK1-cofilin regulate a population of actin filaments required for dynamin-syndapin-cortactin-dependent generation and/or fission of precursors to p75 transporters.
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