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The role of carbohydrate in recombinant human erythropoietin.
Resistance of erythropoietin to thermal inactivation is largely due to the presence of sugars, and terminal sialic acids greatly contribute to the stability. Expand
Effects of site-directed removal of N-glycosylation sites in human erythropoietin on its production and biological properties.
Erythropoietin has two major functions; N-linked sugars are important for proper biosynthesis and/or secretion and expression of the in vivo activity probably by enhancing survival in the circulation and N-Linked sugars of Epo affect binding affinity of the ligand to the receptor but do not play a key role in expression of in vitro activity. Expand
Nitrogen Distribution and Non-Protein Nitrogenous Compounds in Human and Bovine Milk
1) 日本人の人乳の窒素分布を求めた。粗たん白質含量 (全窒素×6.38) は分べん後の日数につれて, 従来の結果と同様な減少の傾向を示した。2) 全窒素に対するカゼイン態窒素の割合は, 人乳I (分べん後1~10日まで) 8.9%; 人乳II (分べん後10~115日) 37.0%; 牛乳78.0%であった。3) 全窒素に対する乳清たん白質態窒素の割合は, 人乳I 60.5%; 人乳IIExpand
Comparative structural study of N-linked oligosaccharides of urinary and recombinant erythropoietins.
The structures of the N-linked oligosaccharides of the urinary erythropoietin (u-EPO) purified from urine of aplastic anemic patients were analyzed and compared with those for recombinantExpand
Renal anemia in polycystic kidney disease mouse.
It was suggested that the anemia in DBA/2FG-pcy mice was due to the disorder of erythropoietin production caused by the progressive polycystic formation in kidneys. Expand
Central muscarinic activities of an M1-selective agonist: preferential effect on reversal of amnesia
Results show that the selective M1 agonist FKS-508 differentiates highly between the central muscarinic effects. Expand
Effects of Salicylate and Other Enhancers on Rectal Absorption of Erythropoietin in Rats
Abstract— To develop a new treatment for patients with anaemia in which erythropoietin (EPO) can be given without injection, the effects of promoters of the rectal absorption of EPO were studied.Expand
The structure of SN-07 chromophore.
Reconstruction of the Macromolecular Antitumor Antibiotic SN-07 from SN-07 Chromophore and DNA
To characterize the novel macromolecular antitumor antibiotic SN-07 and the role of DNA in SN-07, we reconstructed SN-07 in vitro from SN-07 chromophore and calf thymus DNA. Reconstructed SN-07 wasExpand
Production of Recombinant Human Erythropoietin in Mammalian Cells: Host–Cell Dependency of the Biological Activity of the Cloned Glycoprotein
The results indicated that the carbohydrates attached to the EPO peptide are responsible for the different biological activities of these proteins. Expand