Further studies on the zone-strip technique
Micro-quantitative analysis by the zone-strip technique
Identification of an intramitochondrially synthesized proteolipid associated with the mitochondrial ATPase complex as the product of a mitochondrial gene determining oligomycin resistance in…
- M. Marahiel, G. Imam, P. Nelson, N. Pieniązek, P. Stepien, H. Küntzel
- Biology, ChemistryEuropean Journal of Biochemistry
- 1 June 1977
An extranuclear oligomycin-resistant mutant of Aspergillus nidulans was isolated and biochemically analyzed and it was concluded that this small lipophilic protein is coded by a mitochondrial gene determining oligomecin resistance, is synthesized on mitochondrial ribosomes and is associated with the membrane sector of the ATPase complex.
Mitochondrial ATPase complex of Aspergillus nidulans and the dicyclohexylcarbodiimide-binding protein.
Using a double-labelling technique in the absence and presence of cycloheximide, followed by immunoprecipitation of the enzyme complex with antiserum against Neuospora crassa F1 ATPase, it has been shown that this subunit is synthesized on cytoplasmic ribosomes.
Biological breakdown of benzylpenicillin by preformed mats of penicillin-producing organisms.
Penicillium chrysogenum and Aspergillus flavus degraded benzylpenicillin in the same way andCatabolism of valine by the preformed mats of the two moulds confirms this result.
Analytical results for the paper-chromatographic zone-strip technique.
ASK-753, a new iron-containing antibiotic.
A new iron-containing peptide which inhibits the growth of Gram-positive and some Gram-negative bacteria was isolated from the broth of Streptomyces AS-K-753, which is similar in many properties to the sideromycin but differs from it in certain crucial characteristics.
Further studies on ASN-136 and monoketo-organomycin cystaurimycin, a broad spectrum substance produced by partial enzymic digestion of monoketo-organomycin.
The two antibiotics ASN-136 and monoketo-organomycin showed very close similarities in their UV, IR spectra and elemental analysis to those of tuberactinomycin and yazumycin respectively, which revealed the novelty of the two former antibiotics.
Negapillin, a new antibiotic isolated from metabolites of Penicillium chrysogenum.
The determination of compounds by use of chromatography on thin-layer chromatostrips.
A relationship between the length, L, of the zone occupied by a compound, the weight, C, of compound applied and the distance, D, travelled by the developing solvent, has been found to hold under specified conditions and can be used to determine organic compounds alone or in admixture.