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Size-distribution analysis of proteins by analytical ultracentrifugation: strategies and application to model systems.
TLDR
It is shown that the least-squares approach ls-g*(s) can be extrapolated to infinite time by considering area divisions analogous to boundary divisions in the van Holde-Weischet method, thus allowing the transformation of interference optical data into an integral sedimentation coefficient distribution G(s). Expand
C-terminal domain of the membrane copper transporter Ctr1 from Saccharomyces cerevisiae binds four Cu(I) ions as a cuprous-thiolate polynuclear cluster: sub-femtomolar Cu(I) affinity of three
TLDR
The results provide quantitative support for the proposals that, in yeast, (a) "free" copper concentrations are very low in the cytosol and (b) the Cu(I) trafficking gradient is shallow along the putative Ctrlc --> Atx1 --> Ccc2n metabolic pathway. Expand
Analytical ultracentrifugation for the study of protein association and assembly.
TLDR
New applications of tracer sedimentation equilibrium experiments and the development of a fluorescence optical system for the analytical ultracentrifuge extend the accessible concentration range over several orders of magnitude and, coupled with the new analytical procedures, provide powerful new tools for studies of both weak and strong macromolecular interactions in solution. Expand
High affinity interleukin-6 receptor is a hexameric complex consisting of two molecules each of interleukin-6, interleukin-6 receptor, and gp-130.
TLDR
Using a combination of size-exclusion chromatography and analytical ultracentrifugation analysis, in the low affinity receptor complex, IL-6 was shown to bind sIL-6R in a stoichiometric ratio of 1:1, whereas the high affinity ternary complex is hexameric consisting of two molecules each of IL- 6, sIL -6R, and sgp-130. Expand
PcoE--a metal sponge expressed to the periplasm of copper resistance Escherichia coli. Implication of its function role in copper resistance.
TLDR
The properties of PcoE are consistent with a role for P coE as a 'metal sponge' acting as a first line of defence against metal toxicity (under the control of the copper tolerance system cusRS) until the copper resistance operon pcoABCD is expressed. Expand
High prealbumin and transferrin mRNA levels in the choroid plexus of rat brain.
TLDR
The results obtained suggest very active synthesis of prealbumin in choroid plexus, which would be an important link in the transport of thyroid hormones from the blood to the brain via the cerebrospinal fluid. Expand
Fibrillar Amyloid Protein Present in Atheroma Activates CD36 Signal Transduction*
TLDR
This work shows that apolipoprotein C-II, a component of very low and high density lipoproteins, readily forms amyloid fibrils that initiate macrophage inflammatory responses including reactive oxygen production and tumor necrosis factor α expression, and characterize a CD36-dependent signaling cascade initiated by fibrillar amyloids species that may promote atherogenesis. Expand
Self-association of Human Apolipoprotein E3 and E4 in the Presence and Absence of Phospholipid*
TLDR
Results reveal that apoE oligomers undergo phospholipid-induced dissociation to folded monomers, suggesting the monomeric form prevails on the lipoprotein surface in vivo. Expand
Histidine-13 is a crucial residue in the zinc ion-induced aggregation of the A beta peptide of Alzheimer's disease.
TLDR
This work uses circular dichroism spectroscopy, sedimentation assays, and immobilized metal ion affinity chromatography to show that the substitution of Arg for His-13 is responsible for the different Zn(2+)-induced aggregation behavior of rat and human Abeta. Expand
Rat transthyretin (prealbumin). Molecular cloning, nucleotide sequence, and gene expression in liver and brain.
TLDR
By synthesizing an important hormone carrier protein, the choroid plexus may be an important link in the chemical communication between the central nervous system and the bloodstream. Expand
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