Share This Author
Nucleic Acids Research
The results suggest that overall polymerase activity fluctuates with a peak occurring at approximately early S phase, which indicates that DNA polymerase I is subject to a complex control and imply that it has a role in both DNA synthesis and DNA repair.
Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
The use of deoxyadenosine 5'-(alpha-[35S]thio)triphosphate as the label incorporated in dideoxynucleotide sequence reactions increases the sharpness of the bands on an autoradiograph and so increases the resolution achieved.
Nucleotide sequence of bacteriophage lambda DNA.
Modulating DNA bending affects NodD-mediated transcriptional control in Rhizobium leguminosarum
- Xiao-Chun Chen, Jie Feng, Bihe Hou, Fengqing Li, Qiang Li, G. Hong
- BiologyNucleic Acids Research
- 4 May 2005
It is shown that the nod gene inducer naringenin decreased gel mobility of nod box DNA–NodD complexes by sharpening the NodD-induced DNA bend, which correlated with nodA transcription activation.
Secretion of the Rhizobium leguminosarum nodulation protein NodO by haemolysin‐type systems
- A. Scheu, A. Economou, G. Hong, S. Ghelani, A. Johnston, J. Downie
- BiologyMolecular microbiology
- 1 January 1992
Several rhizobia and an Agrobacterium strain containing the cloned nodO gene were found to have the ability to secrete NodO, indicating that a wide range of the Rhizobiaceae have a protein secretion mechanism analogous to that which secretes haemotysin and related toxins and proteases in the Enterobacteriaceae.
Inactivation of the nod box distal half-site allows tetrameric NodD to activate nodA transcription in an inducer-independent manner.
Mutation of the inverted repeat of the nod box distal half-site allowed NodD to activate nodA transcription in an inducer-independent manner in vivo, and to modulate the DNA bending of the Nod D-nod box complex in the absence of inducer in vitro.
A systemic DNA sequencing strategy.
- G. Hong
- Biology, ChemistryJournal of molecular biology
- 5 July 1982
APPENDIX II Complete Annotated Lambda Sequence
Here the authors present the completely annotated λ DNA sequence, where the four-character designation is indicated above the numbered base, and the complete annotation is repeated below the line of sequence.
In Rhizobium leguminosarum, NodD represses its own transcription by competing with RNA polymerase for binding sites.
It is shown that negative autoregulation of nodD was achieved through competition between RNAP and NodD for their respective binding sites, which largely overlap.
Heat-stable DNA polymerase I large fragment resolves hairpin structure in DNA sequencing.
The heat-stable large fragment obtained has proved to be the most active at 65 degrees C and when the sequencing reaction was carried out at this temperature, the hairpin structure was resolved and the sequencing gels obtained were satisfactory.