• Publications
  • Influence
SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor
TLDR
It is demonstrated that SARS-CoV-2 uses the SARS -CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming, and it is shown that the sera from convalescent SARS patients cross-neutralized Sars-2-S-driven entry.
Genomic Characterization of Severe Acute Respiratory Syndrome-Related Coronavirus in European Bats and Classification of Coronaviruses Based on Partial RNA-Dependent RNA Polymerase Gene Sequences
TLDR
An evaluation of CoV in rhinolophid and vespertilionid bat species common in Europe found that proteins 3b and 6 were highly divergent from those proteins in all known SARS-related CoV, highlighting the importance of assessments of the zoonotic potential of widely distributed bat-borne CoV.
Bats host major mammalian paramyxoviruses
TLDR
Major discoveries include evidence of an origin of Hendra- and Nipah virus in Africa, identification of a bat virus conspecific with the human mumps virus, detection of close relatives of respiratory syncytial virus, mouse pneumonia- and canine distemper virus in bats, as well as direct evidence of Sendai virus in rodents.
Select what you need: a comparative evaluation of the advantages and limitations of frequently used expression systems for foreign genes.
TLDR
The most frequently used prokaryotic, yeast, insect and mammalian expression systems, as well as expression in eukaryote individuals are reviewed and the merits and demerits of these systems are discussed.
The SARS-Coronavirus-Host Interactome: Identification of Cyclophilins as Target for Pan-Coronavirus Inhibitors
TLDR
Overexpression of NSP1 and infection with live SARS-CoV strongly increased signalling through the Calcineurin/NFAT pathway and enhanced the induction of interleukin 2, compatible with late-stage immunopathogenicity and long-term cytokine dysregulation as observed in severe SARS cases.
The S protein of bovine coronavirus is a hemagglutinin recognizing 9-O-acetylated sialic acid as a receptor determinant
TLDR
The S protein of bovine coronavirus has been isolated from the viral membrane and purified by gradient centrifugation and the potential of S protein as a probe for the detection of Neu5,9Ac2-containing glycoconjugates is demonstrated.
Point mutations in the S protein connect the sialic acid binding activity with the enteropathogenicity of transmissible gastroenteritis coronavirus
TLDR
It is shown that TGEV virions with efficient hemagglutinating activity were also obtained when cells were treated with sialidase prior to infection, which connects sialic acid binding activity with the enteropathogenicity of T GEV.
Transmissible gastroenteritis coronavirus, but not the related porcine respiratory coronavirus, has a sialic acid (N-glycolylneuraminic acid) binding activity
TLDR
The hemagglutinating activity of transmissible gastroenteritis virus (TGEV), an enteric porcine coronavirus, was analyzed and found to be dependent on the presence of alpha-2,3-linked sialic acid on the erythrocyte surface, suggesting that the sIALic acid binding site is blocked by virus-associated competitive inhibitors.
Structural and Functional Analysis of the Surface Protein of Human Coronavirus OC43
TLDR
A high degree of sequence homology was found between the S proteins of HCV-OC43 and bovine coronavirus and there are subtle differences in the affinity of both viruses for 9-O-acetylated sialic acid.
The Hemagglutinin of Canine Distemper Virus Determines Tropism and Cytopathogenicity
TLDR
The fusogenicity, growth characteristics, and tropism of the recombinant viruses were examined and compared with those of the parental strains and all these characteristics were found to be predominantly mediated by the H protein regardless of the viral backbone used.
...
1
2
3
4
5
...