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New use of BCG for recombinant vaccines
Extrachromosomal and integrative expression vectors carrying the regulatory sequences for major BCG heat-shock proteins have been developed and can elicit long-lasting humoral and cellular immune responses to foreign antigens in mice. Expand
DNA sequence and expression of the B95-8 Epstein—Barr virus genome
The complete (172,282 base pairs) nucleotide sequence of the B95-8 strain of Epstein–Barr virus has been established using the dideoxynucleotide/M13 sequencing procedure. Many RNA polymerase IIExpand
Recombineering in Mycobacterium tuberculosis
Genetic dissection of M. tuberculosis is complicated by its slow growth and its high rate of illegitimate recombination relative to homologous DNA exchange. We report here the development of a facileExpand
Evolutionary relationships among diverse bacteriophages and prophages: all the world's a phage.
DNA and predicted protein sequence similarities, implying homology, are reported, among genes of double-stranded DNA (dsDNA) bacteriophages and prophages spanning a broad phylogenetic range of host bacteria, suggesting common ancestry among these phage genes. Expand
Origins of Highly Mosaic Mycobacteriophage Genomes
The genomic sequences of ten newly isolated mycobacteriophages suggest that the bacteriophage population as a whole is amazingly diverse and may represent the largest unexplored reservoir of sequence information in the biosphere. Expand
Genomic sequences of bacteriophages HK97 and HK022: pervasive genetic mosaicism in the lambdoid bacteriophages.
The complete genome DNA sequences of HK97 and HK022, double-stranded DNA bacteriophages of Escherichia coli and members of the lambdoid or lambda-like group of phages, are reported and a new class of genetic elements, the morons, are identified, which consist of a protein-coding region flanked by aPutative delta 70 promoter and a putative factor-independent transcription terminator. Expand
Growth of Mycobacterium tuberculosis biofilms containing free mycolic acids and harbouring drug-tolerant bacteria
It is shown that M. tuberculosis forms biofilms with specific environmental and genetic requirements distinct from those for planktonic growth, which contain an extracellular matrix rich in free mycolic acids, and harbour an important drug‐tolerant population that persist despite exposure to high levels of antibiotics. Expand
GroEL1: A Dedicated Chaperone Involved in Mycolic Acid Biosynthesis during Biofilm Formation in Mycobacteria
Mycobacteria are unusual in encoding two GroEL paralogs, GroEL1 and GroEL2, which modulates synthesis of mycolates during biofilm formation and physically associates with KasA, a key component of the type II Fatty Acid Synthase involved in mycolic acid synthesis. Expand
Bacteriophage Mu genome sequence: analysis and comparison with Mu-like prophages in Haemophilus, Neisseria and Deinococcus.
Comparisons to eight complete or nearly complete Mu-like prophage genomes found in the genomes of a diverse group of bacteria confirm that members of the Mu-related family of phage genomes are genetically mosaic with respect to each other, and show that the Mu head assembly scaffolding protein is encoded by a gene nested in-frame within the C-terminal half of another gene that encodes the putative head maturation protease. Expand
Efficient site-specific integration in Plasmodium falciparum chromosomes mediated by mycobacteriophage Bxb1 integrase
An efficient, site-specific system of genetic integration into Plasmodium falciparum malaria parasite chromosomes mediated by mycobacteriophage Bxb1 integrase is reported, which illustrates the utility of Bxa1-based integrative recombination for genetic studies of intracellular eukaryotic organisms. Expand