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Discrete Small RNA-Generating Loci as Master Regulators of Transposon Activity in Drosophila
TLDR
Examination of piwi-interacting RNAs associated with each Drosophila Piwi protein finds that Piwi and Aubergine bind RNAs that are predominantly antisense to transposons, whereas Ago3 complexes contain predominantly sense piRNAs. Expand
Role for a bidentate ribonuclease in the initiation step of RNA interference
TLDR
Dicer is a member of the RNase III family of nucleases that specifically cleave double-stranded RNAs, and is evolutionarily conserved in worms, flies, plants, fungi and mammals, and has a distinctive structure, which includes a helicase domain and dualRNase III motifs. Expand
An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells
TLDR
It is shown that ‘loss-of-function’ phenotypes can be created in cultured Drosophila cells by transfection with specific double-stranded RNAs, which coincides with a marked reduction in the level of cognate cellular messenger RNAs. Expand
Rb-Mediated Heterochromatin Formation and Silencing of E2F Target Genes during Cellular Senescence
TLDR
A distinct heterochromatic structure that accumulates in senescent human fibroblasts is described, which is designated senescence-associated heterochROMatic foci (SAHF) and is associated with the stable repression of E2F target genes. Expand
Argonaute2 Is the Catalytic Engine of Mammalian RNAi
TLDR
The evidence supports a model in which Argonaute contributes “Slicer” activity to RISC, providing the catalytic engine for RNAi. Expand
Landscape of transcription in human cells
TLDR
Evidence that three-quarters of the human genome is capable of being transcribed is reported, as well as observations about the range and levels of expression, localization, processing fates, regulatory regions and modifications of almost all currently annotated and thousands of previously unannotated RNAs that prompt a redefinition of the concept of a gene. Expand
A new regulatory motif in cell-cycle control causing specific inhibition of cyclin D/CDK4
TLDR
P16 seems to act in a regulatory feedback circuit with CDK4, D-type cyclins and retinoblastoma protein, and inhibits the catalytic activity of theCDK4/cyclin D enzymes. Expand
Specialized piRNA Pathways Act in Germline and Somatic Tissues of the Drosophila Ovary
TLDR
It is found that distinct piRNA pathways with differing components function in ovarian germ and somatic cells, demonstrating that the architecture of piRNA clusters has coevolved with the transposons that they are tasked to control. Expand
Sorting of Small RNAs into Arabidopsis Argonaute Complexes Is Directed by the 5′ Terminal Nucleotide
TLDR
A cataloged small RNAs resident in four AGO complexes found that AGO2 and AGO4 preferentially recruit smallRNAs with a 5' terminal adenosine, whereas AGO1 harbors microRNAs (miRNAs) that favor a5' terminal uridine, perhaps explaining the evolutionary drive for miRNAs to initiate with uridine. Expand
Argonaute2, a Link Between Genetic and Biochemical Analyses of RNAi
TLDR
Biochemical purification of the RNAi effector nuclease from cultured Drosophila cells reveals that one constituent of this complex is a member of the Argonaute family of proteins, which are essential for gene silencing in Caenorhabditis elegans, Neurospora, and Arabidopsis. Expand
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