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Chromatin accessibility pre-determines glucocorticoid receptor binding patterns
TLDR
Up to 95% of de novo genomic binding by the glucocorticoid receptor, a paradigmatic ligand-activated transcription factor, is targeted to preexisting foci of accessible chromatin, defining a framework for understanding regulatory factor–genome interactions and providing a molecular basis for the tissue selectivity of steroid pharmaceuticals and other agents that intersect the living genome. Expand
Global Nature of Dynamic Protein-Chromatin Interactions In Vivo: Three-Dimensional Genome Scanning and Dynamic Interaction Networks of Chromatin Proteins
TLDR
It is demonstrated that most chromatin proteins have a high turnover on chromatin with a residence time on the order of seconds, that the major fraction of each protein is bound to chromatin at steady state, and that transient binding is a common property of chromatin-associated proteins. Expand
Extensive chromatin remodelling and establishment of transcription factor ‘hotspots’ during early adipogenesis
TLDR
This work has employed DNase I hypersensitive site analysis to investigate the genome‐wide changes in chromatin structure that accompany the binding of adipogenic transcription factors and demonstrates that C/EBPβ marks a large number of transcription factor ‘hotspots’ before induction of differentiation and chromatin remodelling and is required for their establishment. Expand
The glucocorticoid receptor: rapid exchange with regulatory sites in living cells.
TLDR
Photobleaching experiments provide direct evidence that the hormone-occupied receptor undergoes rapid exchange between chromatin and the nucleoplasmic compartment, suggesting that the interaction of regulatory proteins with target sites in chromatin is a more dynamic process than previously believed. Expand
NSD1 is essential for early post‐implantation development and has a catalytically active SET domain
TLDR
The nuclear receptor‐binding SET domain‐containing protein (NSD1) belongs to an emerging family of proteins, which have all been implicated in human malignancy, and it is found that its SET domain possesses intrinsic histone methyltransferase activity with specificity for Lys36 of hist one H3 (H3‐K36) and Lys20 of histone H4 (H4‐K20). Expand
Fluorescence resonance energy transfer from cyan to yellow fluorescent protein detected by acceptor photobleaching using confocal microscopy and a single laser
TLDR
An extensively characterize a simple approach to FRET that should be adaptable to most laser‐scanning confocal microscopes, and demonstrate its feasibility for detecting FRET between several CFP/YFP partners. Expand
Ultradian hormone stimulation induces glucocorticoid receptor-mediated pulses of gene transcription
TLDR
It is demonstrated that ultradian hormone stimulation induces cyclic GR-mediated transcriptional regulation, or gene pulsing, both in cultured cells and in animal models, indicating that biologically accurate regulation of gene targets by GR requires an ultradian mode of hormone stimulation. Expand
eRNAs promote transcription by establishing chromatin accessibility at defined genomic loci.
TLDR
The data suggest that eRNAs contribute to establishing a cell-type-specific transcriptional circuitry by directing chromatin-remodeling events by regulating genomic access of the transcriptional complex to defined regulatory regions. Expand
Early Th1 cell differentiation is marked by a Tfh cell-like transition.
TLDR
This work showed interleukin-12 acting via the transcription factor STAT4 induced both Il21 and Bcl6 genes, generating cells with features of both Tfh and Th1 cells, however, STAT4 also induced the transcription factors T-bet, which represses TfH cell functionalities, promoting full Th1 cell differentiation. Expand
Sequence‐specific positioning of nucleosomes over the steroid‐inducible MMTV promoter.
TLDR
The results indicate that this region is associated in chromatin from uninduced cells with a macromolecular complex (probably a nucleosome core), and this complex is displaced (or modified) upon binding of activated receptor. Expand
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