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Sperm decondensation in Xenopus egg cytoplasm is mediated by nucleoplasmin
At fertilization, sperm chromatin decondenses in two stages, which can be mimicked in extracts of Xenopus eggs. Rapid, limited decondensation is followed by slower, membrane-dependent decondensationExpand
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Nucleoplasmin remodels sperm chromatin in Xenopus egg extracts
Nucleoplasmin is necessary and sufficient for the initial stage of Xenopus sperm decondensation in egg extracts. In this article we show that sperm decondensation is accompanied by loss of twoExpand
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Unfolding individual nucleosomes by stretching single chromatin fibers with optical tweezers
Single chromatin fibers were assembled directly in the flow cell of an optical tweezers setup. A single λ phage DNA molecule, suspended between two polystyrene beads, was exposed to a Xenopus laevisExpand
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The nuclear membrane prevents replication of human G2 nuclei but not G1 nuclei in Xenopus egg extract
We have used synchronized HeLa cells to investigate the role of the nuclear membrane in preventing rereplication in a single cell cycle. Nuclei were prepared with intact nuclear membranes usingExpand
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Cloned ferrets produced by somatic cell nuclear transfer.
Somatic cell nuclear transfer (SCNT) offers great potential for developing better animal models of human disease. The domestic ferret (Mustela putorius furo) is an ideal animal model for influenzaExpand
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Hyperphosphorylation of Nucleoplasmin Facilitates Xenopus Sperm Decondensation at Fertilization (*)
Previous studies showed that the nuclear phosphoprotein nucleoplasmin performs the first stage of chromatin decondensation of Xenopus sperm at fertilization. It binds and removes sperm basic proteinsExpand
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Adeno-associated virus-targeted disruption of the CFTR gene in cloned ferrets.
Somatic cell gene targeting combined with nuclear transfer cloning presents tremendous potential for the creation of new, large-animal models of human diseases. Mouse disease models often fail toExpand
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Identification of Differentially Expressed Genes in Individual Bovine Preimplantation Embryos Produced by Nuclear Transfer: Improper Reprogramming of Genes Required for Development1
Abstract Using an interwoven-loop experimental design in conjunction with highly conservative linear mixed model methodology using estimated variance components, 18 genes differentially expressedExpand
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The roles of nucleolar structure and function in the subcellular location of the HIV-1 Rev protein.
The human immunodeficiency virus 1 (HIV-1) Rev transactivator protein plays a critical role in the regulation of expression of structural proteins by controlling the pathway of mRNA transport. TheExpand
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Efficient Term Development of Vitrified Ferret Embryos Using a Novel Pipette Chamber Technique1
Abstract Development of an efficient cryopreservation technique for the domestic ferret is key for the long-term maintenance of valuable genetic specimens of this species and for the conservation ofExpand
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