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EB1 and APC bind to mDia to stabilize microtubules downstream of Rho and promote cell migration
Results show an evolutionarily conserved pathway for microtubule capture, and suggest that mDia functions as a scaffold protein for EB1 and APC to stabilize microtubules and promote cell migration. Expand
Microtubule-induced focal adhesion disassembly is mediated by dynamin and focal adhesion kinase
This work develops a model system of focal adhesion disassembly based on the finding that microtubule regrowth after nocodazole washout induces disassembly of focalAdhesion, and that this disassembly occurs independently of Rho and Rac, but depends on focaladhesion kinase (FAK) and dynamin. Expand
mDia mediates Rho-regulated formation and orientation of stable microtubules
The results show that mDia is sufficient to generate and orient stable microtubules, and indicate that Dia-related formins are part of a conserved pathway that regulates the dynamics of microtubule ends. Expand
Nuclear Movement Regulated by Cdc42, MRCK, Myosin, and Actin Flow Establishes MTOC Polarization in Migrating Cells
The results show that nuclear repositioning is an initial polarizing event in migrating cells and that the positions of the nucleus and the MTOC are established by separate regulatory pathways. Expand
The formin mDia2 stabilizes microtubules independently of its actin nucleation activity
This work generated two actin mutants in a constitutively active version of mDia2 containing formin homology domains 1 and 2 and found that they still induced stable MTs and bound to the MT TIP proteins EB1 and APC, which have also been implicated in MT stabilization. Expand
Clathrin mediates integrin endocytosis for focal adhesion disassembly in migrating cells
Results show that focal adhesion disassembly occurs through a targeted mechanism involving MTs, clathrin, and specificClathrin adaptors and that direct endocytosis of integrins from focal adhesions mediates their disassembly in migrating cells. Expand
Localized Stabilization of Microtubules by Integrin- and FAK-Facilitated Rho Signaling
It is reported that integrin-mediated activation of focal adhesion kinase (FAK) at the leading edge is required for MT stabilization by the Rho-mDia signaling pathway in mouse fibroblasts. Expand
Linear Arrays of Nuclear Envelope Proteins Harness Retrograde Actin Flow for Nuclear Movement
The coupling of actin cables to the nuclear membrane for nuclear movement via specific membrane proteins indicates that, like plasma membrane integrins, nuclear membrane proteins assemble into actin-dependent arrays for force transduction. Expand
Cdc42, dynein, and dynactin regulate MTOC reorientation independent of Rho-regulated microtubule stabilization
It is shown that serum and the serum lipid, lysophosphatidic acid (LPA), increased Cdc42 GTP levels and triggeredMTOC reorientation in serum-starved wounded monolayers of 3T3 fibroblasts, establishing an LPA/Cdc42 signaling pathway that regulates MTOC re orientation in a dynein-dependent manner. Expand
Distinct populations of microtubules: Tyrosinated and nontyrosinated alpha tubulin are distributed differently in vivo
A unique post-translational modification of tubulin has previously been described in which a tyrosine residue is reversibly added to the C terminus of the alpha-tubulin subunit. We have preparedExpand