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The complete genome sequence of the Gram-positive bacterium Bacillus subtilis
Bacillus subtilis is the best-characterized member of the Gram-positive bacteria. Its genome of 4,214,810 base pairs comprises 4,100 protein-coding genes. Of these protein-coding genes, 53% areExpand
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Characterization and identification of vaccine candidate proteins through analysis of the group A Streptococcus surface proteome
We describe a proteomic approach for identifying bacterial surface-exposed proteins quickly and reliably for their use as vaccine candidates. Whole cells are treated with proteases to selectivelyExpand
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Previously unrecognized vaccine candidates against group B meningococcus identified by DNA microarrays
We have used DNA microarrays to follow Neisseria meningitidis serogroup B (MenB) gene regulation during interaction with human epithelial cells. Host-cell contact induced changes in the expression ofExpand
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Sortase A Utilizes an Ancillary Protein Anchor for Efficient Cell Wall Anchoring of Pili in Streptococcus agalactiae
ABSTRACT Pili are putative virulence factors and promising vaccine candidates in Streptococcus agalactiae (group B Streptococcus [GBS]) infection, a leading cause of neonatal sepsis and meningitis.Expand
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Structure analysis and site‐directed mutagenesis of defined key residues and motives for pilus‐related sortase C1 in group B Streptococcus
In group B Streptococcus (GBS), 3 structurally distinct types of pili have been discovered as potential virulence factors and vaccine candidates. The pilus‐forming proteins are assembled intoExpand
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Surfome Analysis as a Fast Track to Vaccine Discovery
Safe recombinant vaccines, based on a small number of antigenic proteins, are emerging as the most attractive, cost-effective solution against infectious diseases. In the present work, we confirmedExpand
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Group B Streptococcus crosses human epithelial cells by a paracellular route.
Colonization of the colon and vagina is thought to be important in the pathogenesis of group B Streptococcus (GBS) infection. However, little is known about the strategies used by GBS to translocateExpand
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Functional Characterization of a Newly Identified Group B Streptococcus Pullulanase Eliciting Antibodies Able to Prevent Alpha-Glucans Degradation
Streptococcal pullulanases have been recently proposed as key components of the metabolic machinery involved in bacterial adaptation to host niches. By sequence analysis of the Group B StreptococcusExpand
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Capturing host‐pathogen interactions by protein microarrays: identification of novel streptococcal proteins binding to human fibronectin, fibrinogen, and C4BP
Microbial pathogen entry and survival in the host is mediated by a network of molecular interactions between the two partners, which has been the subject of many research efforts. A complex pictureExpand
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Targeted Amino Acid Substitutions Impair Streptolysin O Toxicity and Group A Streptococcus Virulence
ABSTRACT Streptolysin O is a potent pore-forming toxin produced by group A Streptococcus. The aims of the present study were to dissect the relative contributions of different structural domains ofExpand
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