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Improvement of Escherichia coli production strains by modification of the phosphoenolpyruvate:sugar phosphotransferase system
  • G. Gosset
  • Biology, Medicine
  • Microbial cell factories
  • 16 May 2005
The application of metabolic engineering in Escherichia coli has resulted in the generation of strains with the capacity to produce metabolites of commercial interest. Biotechnological processes withExpand
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Analysis of bacterial community during the fermentation of pulque, a traditional Mexican alcoholic beverage, using a polyphasic approach.
In this study, the characterization of the bacterial community present during the fermentation of pulque, a traditional Mexican alcoholic beverage from maguey (Agave), was determined for the firstExpand
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Metabolic engineering and protein directed evolution increase the yield of L‐phenylalanine synthesized from glucose in Escherichia coli
L‐phenylalanine (L‐Phe) is an aromatic amino acid with diverse commercial applications. Technologies for industrial microbial synthesis of L‐Phe using glucose as a starting raw material currentlyExpand
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Metabolic engineering for the production of shikimic acid in an evolved Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system
BackgroundShikimic acid (SA) is utilized in the synthesis of oseltamivir-phosphate, an anti-influenza drug. In this work, metabolic engineering approaches were employed to produce SA in EscherichiaExpand
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Response to different environmental stress conditions of industrial and laboratory Saccharomyces cerevisiae strains
Two sets of Saccharomyces cerevisiae strains were compared for their physiological responses to different stress conditions. One group is composed of three strains adapted to controlled laboratoryExpand
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Engineering Escherichia coli to overproduce aromatic amino acids and derived compounds
The production of aromatic amino acids using fermentation processes with recombinant microorganisms can be an advantageous approach to reach their global demands. In addition, a large array ofExpand
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Characterization of sugar mixtures utilization by an Escherichia coli mutant devoid of the phosphotransferase system
Abstract. Due to catabolite repression in microorganisms, sugar mixtures cannot be metabolized in a rapid and efficient manner. Therefore, the development of mutant strains that avoid this regulatoryExpand
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Transcriptional and metabolic response of recombinant Escherichia coli to spatial dissolved oxygen tension gradients simulated in a scale-down system.
Escherichia coli, expressing recombinant green fluorescent protein (GFP), was subjected to dissolved oxygen tension (DOT) oscillations in a two-compartment system for simulating gradients that canExpand
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Utility of an Escherichia coli strain engineered in the substrate uptake system for improved culture performance at high glucose and cell concentrations: An alternative to fed‐batch cultures
Overflow metabolism is an undesirable characteristic of aerobic cultures of Escherichia coli. It results from elevated glucose consumption rates that cause a high substrate conversion to acetate,Expand
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Culture of Escherichia coli under dissolved oxygen gradients simulated in a two-compartment scale-down system: metabolic response and production of recombinant protein.
A significant problem of large-scale cultures, but scarcely studied for recombinant E. coli, is the presence of gradients in dissolved oxygen tension (DOT). In this study, the effect of DOT gradientsExpand
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