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Measurement of protein in cell suspensions using the Coomassie brilliant blue dye-binding assay.
Abstract The direct measurement of protein in cell suspensions using the Coomassie brilliant blue dye-binding assay demonstrated markedly lower values compared to those obtained with the Lowry assay.Expand
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Comparison of three D-dimer assays for the diagnosis of DVT:ELISA, latex and an immunofiltration assay (NycoCard D-Dimer).
Ninety-two consecutive patients referred for suspicion of deep venous thrombosis (DVT) were analyzed for D-dimer using ELISA, latex test, and a new immunofiltration method (NycoCard D-Dimer).Expand
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A method for the isolation of a-granules from human platelets
Abstract A method for the isolation of α-granules is described. A two-step French pressure cell homogenization procedure which directly produced an organelle concentrate suited for loading on densityExpand
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Rapid immunometric measurement of C-reactive protein in whole blood.
We examined an instrument-free test for C-reactive protein (CRP) in whole blood. The NycoCard CRP Whole Blood test uses a cell-solubilizing dilution liquid, a membrane-bound antibody that binds CRP,Expand
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Evidence for release of soluble, but not of membrane-integrated, proteins from human platelet alpha-granules.
Proteins released from stimulated platelets were compared to those of a well-defined preparation of alpha-granules and the soluble cytoplasm by crossed immunoelectrophoresis. Nearly all releasableExpand
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Assay of D-dimer based on immunofiltration and staining with gold colloids.
In this immunofiltration assay of D-dimer in plasma samples, the antigens are captured by a monoclonal antibody on a porous membrane, and labeled with the same antibody conjugated to gold colloids.Expand
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Rapid Method for Detection of Gram-Positive and -Negative Bacteria in Milk from Cows with Moderate or Severe Clinical Mastitis
ABSTRACT A rapid method for demonstration of gram-positive and gram-negative bacteria in milk is described. The technique is based on dilution of the sample in a medium, followed by filtrationExpand
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Fibrinogen-binding properties of the human platelet glycoprotein IIb-=IIIa complex: a study using crossed-radioimmunoelectrophoresis.
Fibrinogen-binding platelet proteins have been examined by crossed-immunoelectrophoresis of solubilized, washed platelets followed by the incubation of the immunoplates with /sup 125/I-fibrinogen andExpand
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A radioimmunoassay for thrombospondin, used in a comparative study of thrombospondin, beta-thromboglobulin and platelet factor 4 in healthy volunteers.
A radioimmunoassay was developed for the platelet alpha-granule protein thrombospondin; concentrations of thrombospondin as low as 3 ng ml-1 could be measured. There was no interference from otherExpand
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Involvement of divalent cations in the complex between the platelet glycoproteins IIb and IIIa.
The major immunoprecipitate (No. 16) seen on crossed immunoelectrophoresis of Triton X-100-solubilized platelet proteins against whole platelet antibodies represents a complex containing the membraneExpand
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