• Publications
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Sulfur and selenium: the role of oxidation state in protein structure and function.
It is apparent that the chalcogen amino acids cysteine, methionine, selenocysteines, and selenomethionine exhibit a unique biological chemistry that is the source of exciting research opportunities. Expand
Mycobacterium tuberculosis WhiB3 responds to O2 and nitric oxide via its [4Fe-4S] cluster and is essential for nutrient starvation survival
It is shown that Mtb WhiB3 responds to the dormancy signals NO and O2 through its iron-sulfur (Fe-S) cluster, and EPR analysis demonstrates that NO forms a protein-bound dinitrosyl–iron–dithiol complex with the Fe-S cluster, indicating that NO specifically targets the WhiBs 3Fe-4S cluster. Expand
Xanthine oxidase-dependent regulation of hypoxia-inducible factor in cancer cells.
It is shown that CoCl2 increased xanthine oxidase (XO)-derived reactive oxygen species (ROS), which causes accumulation of HIF1-alpha protein in U251-MG cells, pointing toward a more general role of this transcription factor in tumor progression. Expand
Reactive Sulfur Species: An Emerging Concept in Oxidative Stress
There is mounting evidence that reactive sulfur species (RSS) with stressor properties similar to the ones found in ROS are formed under conditions of oxidative stress, providing new antioxidant drug targets. Expand
Hypothesis: the role of reactive sulfur species in oxidative stress.
It is proposed that an additional group of redox active molecules termed "reactive sulfur species" (RSS) are formed in vivo under conditions of oxidative stress, likely to include disulfide-S-oxides, sulfenic acids, and thiyl radicals, and are predicted to modulate the redox status of biological thiols and disulfides. Expand
Fatty Acid Transduction of Nitric Oxide Signaling
The results reveal that nitroalkene derivatives of linoleic acid are pluripotent signaling mediators that act not only via receptor-dependent mechanisms, but also by transducing the signaling actions of ·NO via pathways subject to regulation by the relative distribution of LNO2 to hydrophobic versus aqueous microenvironments. Expand
Novel method for measuring S-nitrosothiols using hydrogen sulfide.
A novel reaction that is readily adaptable for commercial NO analyzers that utilizes hydrogen sulfide (H2S), a gas that can reduce RSNO to NO and, analogous to NO, is produced by endogenous metabolism and has effects on diverse biological functions. Expand
The redox regulation of thiol dependent signaling pathways in cancer.
  • G. Giles
  • Chemistry, Medicine
  • Current pharmaceutical design
  • 30 November 2006
The mechanisms of ROS generation by the action of oxidoreductases and nitric oxide synthases will be discussed in conjunction with the cell's major antioxidant defenses, with special emphasis placed on the subcellular location of these redox reactions. Expand
Moderate hypoxia induces xanthine oxidoreductase activity in arterial endothelial cells.
Xanthine oxidoreductase (XOR) activity has been previously noted to be responsive to changes in O2 tension. While prior studies have focused on the extremes (0-3% and 95-100%) of O2 tensions, weExpand