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Carbon-13 CP/MAS solid-state NMR and FT-IR spectroscopy of wood cell wall biodegradation
Abstract The study of the degradation carried out by microorganisms on plant cell walls poses several problems in the analysis of the degraded solid materials. In this article this analytical problemExpand
High throughput assay for cytochrome P450 BM3 for screening libraries of substrates and combinatorial mutants.
Control experiments have proven that this method is specifically detecting NADPH oxidation by catalytic turnover of P450 BM3, which opens the way to automated high throughput screening of NAD(P)H-linked enzymatic activity of molecules of pharmacological and biotechnological interest and libraries of random mutants of NAD-H-dependent biocatalysts. Expand
Breakthrough in P450 bioelectrochemistry and future perspectives.
This paper reviews and discusses the recent literature on electrocatalysis of cytochrome P450 enzymes, and highlights the different approaches that have led to an unprecedented advancement of this area of research. Expand
Structural basis for the functional roles of critical residues in human cytochrome p450 aromatase.
The crystal structure of rArom is determined to show that the active site, tertiary, and quaternary structures are identical to those of pArom, the only enzyme known to catalyze the biosynthesis of estrogens from androgens. Expand
Catalytic properties of catechol 1,2-dioxygenase from Acinetobacter radioresistens S13 immobilized on nanosponges.
Catechol 1,2-dioxygenases are iron containing enzymes able to convert catechol into cis,cis-muconate, a precursor of the industrially important compound adipic acid, and was immobilized on beta-cyclodextrins cross-linked with carbonate groups (nanosponges) with a yield of 29 mg of enzyme per gram of support. Expand
Engineering type 1 copper sites in proteins
The use of site‐directed mutagenesis methods has revolutionalized the study of the so‐called type 1 and type 2 copper sites in proteins. In particular our understanding of the relation between theExpand
Engineering the maltose binding protein for reagentless fluorescence sensing.
A mutant of the maltose binding protein (MBP) in which the serine residue at position 337 is replaced by a cysteine residue using site-directed mutagenesis is described, and this strategy of combined genetic and chemical modification to the development of reagentless fluorescence sensing is discussed. Expand
Direct electrochemistry of immobilized human cytochrome P450 2E1.
This communication reports the first electrochemical study of the human P450 2E1 either absorbed or covalently linked to different electrode surfaces, and molecular modeling of the enzyme helped to rationalize the results. Expand
Proton-coupled electron transfer of flavodoxin immobilized on nanostructured tin dioxide electrodes: thermodynamics versus kinetics control of protein redox function.
It is proposed that this slow deprotonation step has the physiological benefit of preventing the undesirable loss of reducing equivalents which results from semiquinone oxidation to quinone. Expand
P450 versus P420: correlation between cyclic voltammetry and visible absorption spectroscopy of the immobilized heme domain of cytochrome P450 BM3.
The cyclic voltabsorptometric experiments showed how, when both the active and inactive forms are present on the PEI film, the inactive P420 species tends to dominate the cyclic voltammetric signal. Expand