Cloning and characterization of an extracellular Ca2+-sensing receptor from bovine parathyroid
The cloning of complementary DNA encoding an extracellular Ca2+ -sensing receptor from bovine parathyroid is reported with pharmacological and functional properties nearly identical to those of the native receptor.
Molecular physiology and pathophysiology of electroneutral cation-chloride cotransporters.
- G. Gamba
- BiologyPhysiological Reviews
- 1 April 2005
This work is a comprehensive review of the knowledge that has evolved in this area and includes molecular biology of each gene, functional properties of identified cotransporters, structure-function relationships, and physiological and pathophysiological roles of each cotranporter.
Molecular cloning, primary structure, and characterization of two members of the mammalian electroneutral sodium-(potassium)-chloride cotransporter family expressed in kidney.
- G. Gamba, A. Miyanoshita, S. Hebert
- BiologyJournal of Biological Chemistry
- 1 July 1994
Northern blot analysis and in situ hybridization indicate that these transporters are expressed predominantly in kidney with an intrarenal distribution consistent with their recognized functional localization, establishing a new family of Na(+)-(K+)-Cl- cotransporters.
Molecular pathogenesis of inherited hypertension with hyperkalemia: The Na–Cl cotransporter is inhibited by wild-type but not mutant WNK4
- F. H. Wilson, K. Kahle, R. Lifton
- BiologyProceedings of the National Academy of Sciences…
- 6 January 2003
It is demonstrated that WNK4 negatively regulates surface expression of NCCT and implicate loss of this regulation in the molecular pathogenesis of an inherited form of hypertension.
Cloning and Characterization of KCC3 and KCC4, New Members of the Cation-Chloride Cotransporter Gene Family*
- D. Mount, A. Mercado, G. Gamba
- Biology, ChemistryJournal of Biological Chemistry
- 4 June 1999
The expression of mouse KCC3 in Xenopus laevis oocytes reveals the expected functional characteristics of a K+Cl− cotransporter: Cl−-dependent uptake of86Rb+ which is strongly activated by cell swelling and weakly sensitive to furosemide.
Regulation of NKCC2 by a chloride-sensing mechanism involving the WNK3 and SPAK kinases
- José Ponce‐Coria, Pedro San-Cristobal, G. Gamba
- BiologyProceedings of the National Academy of Sciences
- 17 June 2008
Together these data reveal a chloride-sensing mechanism that regulates NKCC2 and provide insight into how increases in the level of intracellular chloride in TAL cells, as seen in certain pathological states, could drastically impair renal salt reabsorption.
Roles of the cation–chloride cotransporters in neurological disease
In the nervous system, the intracellular chloride concentration ([Cl−]i) determines the strength and polarity of γ-aminobutyric acid (GABA)-mediated neurotransmission and represents attractive therapeutic targets in neurological disorders the pathogenesis of which involves deranged cellular chloride homoestasis.
Identification and functional characterization of cation-chloride cotransporters in plants.
- J. M. Colmenero-Flores, Germán Martínez, M. Talón
- Environmental ScienceThe Plant Journal
- 21 March 2007
Analysis of At CCC promoter-beta-glucuronidase transgenic Arabidopsis plants revealed preferential expression in the root and shoot vasculature at the xylem/symplast boundary, root tips, trichomes, leaf hydathodes, leaf stipules and anthers, suggesting At C CC involvement in long-distance Cl(-) transport.
Cloning and functional expression of a rat kidney extracellular calcium/polyvalent cation-sensing receptor.
- D. Riccardi, J. Park, W. Lee, G. Gamba, E. Brown, S. Hebert
- BiologyProceedings of the National Academy of Sciences…
- 3 January 1995
In rat kidney, Northern analysis revealed RaKCaR transcripts of 4 and 7 kb, and in situ hybridization showed localization primarily in outer medulla and cortical medullary rays, which provide important insights into the molecular structure of an extracellular Ca2+/polyvalent cation-sensing receptor in rat kidney.
Unique chloride-sensing properties of WNK4 permit the distal nephron to modulate potassium homeostasis
- A. Terker, Chong Zhang, Kayla J. Erspamer, G. Gamba, Chao‐ling Yang, D. Ellison
- BiologyKidney International
- 27 August 2015
It is reported that modest changes in both dietary and plasma potassium affect the thiazide-sensitive sodium-chloride cotransporter, NCC, in vivo, which explains how unique chloride-sensing properties of WNK4 enable kinase mediating effects of potassium on NCC in vivo.
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