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Randomization of genes by PCR mutagenesis.
A modified polymerase chain reaction (PCR) was developed to introduce random point mutations into cloned genes. The modifications were made to decrease the fidelity of Taq polymerase during DNAExpand
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A general purpose RNA-cleaving DNA enzyme.
An in vitro selection procedure was used to develop a DNA enzyme that can be made to cleave almost any targeted RNA substrate under simulated physiological conditions. The enzyme is comprised of aExpand
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The antiquity of RNA-based evolution
All life that is known to exist on Earth today and all life for which there is evidence in the geological record seems to be of the same form — one based on DNA genomes and protein enzymes. Yet thereExpand
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Mutagenic PCR.
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RNA evolution and the origins of life
The evolution of RNA is likely to have played an important role in the very early history of life on Earth but it is doubtful that life began with RNA. Consideration of what came before RNA must takeExpand
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A DNA enzyme that cleaves RNA.
BACKGROUND Several types of RNA enzymes (ribozymes) have been identified in biological systems and generated in the laboratory. Considering the variety of known RNA enzymes and the similarity of DNAExpand
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Mechanism and utility of an RNA-cleaving DNA enzyme.
We previously reported the in vitro selection of a general-purpose RNA-cleaving DNA enzyme that exhibits a catalytic efficiency (kcat/KM) exceeding that of any other known nucleic acid enzymeExpand
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Self-Sustained Replication of an RNA Enzyme
An RNA enzyme that catalyzes the RNA-templated joining of RNA was converted to a format whereby two enzymes catalyze each other's synthesis from a total of four oligonucleotide substrates. TheseExpand
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Mutagenic PCR.
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A 1.7-kilobase single-stranded DNA that folds into a nanoscale octahedron
Molecular self-assembly offers a means of spontaneously forming complex and well-defined structures from simple components. The specific bonding between DNA base pairs has been used in this way toExpand
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