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Metabolic Engineering of Potato Carotenoid Content through Tuber-Specific Overexpression of a Bacterial Mini-Pathway
TLDR
This is the highest carotenoid and beta-carotene content reported for biofortified potato as well as for any of the four major staple foods (the next best event being “Golden Rice 2”, with 31 mcg/g dry weight beta- carotene). Expand
Metabolic engineering of potato tuber carotenoids through tuber-specific silencing of lycopene epsilon cyclase
TLDR
The data suggest that epsilon-cyclization of lycopene is a key regulatory step in potato tuber carotenogenesis, and upon tuber-specific silencing of the corresponding gene, beta-beta-carotenoid levels are increased, and expression of several other genes in the pathway is modified. Expand
Novel carotenoid cleavage dioxygenase catalyzes the first dedicated step in saffron crocin biosynthesis
TLDR
Using in bacterio, in vitro, and in planta functional assays, it is demonstrated that CCD2 is the dioxygenase catalyzing the first dedicated step in saffron crocetin biosynthesis starting from the carotenoid zeaxanthin. Expand
Metabolic engineering of carotenoid biosynthesis in plants.
TLDR
In the past six years many metabolic engineering efforts have been undertaken in plants to enable the use of plants as 'cell factories' for producing specialty carotenoids and to improve plant resistance to abiotic stress. Expand
Silencing of beta-carotene hydroxylase increases total carotenoid and beta-carotene levels in potato tubers
TLDR
Together with epsilon-cyclization of lycopene, beta-carotene hydroxylation is another regulatory step in potato tuber carotenogenesis and is consistent with a prevalent role of CHY2, which is highly expressed in tubers, in the control of this step. Expand
Improved protocol for Agrobacterium mediated transformation of tomato and production of transgenic plants containing carotenoid biosynthetic gene CsZCD
TLDR
The protocol was successfully employed in the production of transgenic Micro-Tom tomato containing the carotenoid biosynthesis CsZCD under constructive promoter and represents a simple, efficient and general means of transforming tomato. Expand
Light Remodels Lipid Biosynthesis in Nannochloropsis gaditana by Modulating Carbon Partitioning between Organelles1[OPEN]
TLDR
The results highlight the high flexibility of lipid biosynthesis in N. gaditana and lay the foundations for a hypothetical mechanism of regulation of primary carbon partitioning by controlling metabolite allocation at the subcellular level. Expand
Transcriptional-Metabolic Networks in β-Carotene-Enriched Potato Tubers: The Long and Winding Road to the Golden Phenotype1[C][W][OA]
TLDR
Through a “guilt-by-profiling” approach, several endogenous genes for carotenoid biosynthesis likely to play a key regulatory role in Golden tubers are identified, which are candidates for manipulations aimed at the further optimization of tuber carOTenoid content. Expand
Of chromoplasts and chaperones.
TLDR
The Or gene of cauliflower induces differentiation of beta-carotene-containing chromoplasts in the (normally non-pigmented) curd tissue, the first time that a gene product controlling chromoplast differentiation is described. Expand
Candidate Enzymes for Saffron Crocin Biosynthesis Are Localized in Multiple Cellular Compartments1[OPEN]
TLDR
It is proposed that the endoplasmic reticulum and cytoplasm function as transit centers for metabolites whose biosynthesis starts in the plastid and are accumulated in the vacuole to a level up to 10% of the stigma dry weight. Expand
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