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Carbohydrate-binding modules: fine-tuning polysaccharide recognition.
The present review summarizes the impact structural biology has had on the understanding of the mechanisms by which CBMs bind to their target ligands. Expand
Glycosyltransferases: structures, functions, and mechanisms.
The expected two-step double-displacement mechanism is rendered less likely by the lack of conserved architecture in the region where a catalytic nucleophile would be expected, and a mechanism involving a short-lived oxocarbenium ion intermediate now seems the most likely, with the leaving phosphate serving as the base. Expand
Structures and mechanisms of glycosyl hydrolases.
The wealth of information provided by the recent structure determinations of many different glycosyl hydrolases shows that the substrate specificity and the mode of action of these enzymes areExpand
Structural and sequence-based classification of glycoside hydrolases.
The diversity of oligo- and polysaccharides provides an abundance of biological roles for these carbohydrates. The enzymes hydrolysing these compounds, the glycoside hydrolases, therefore mediate aExpand
An evolving hierarchical family classification for glycosyltransferases.
This evolving classification rationalises structural and mechanistic investigation, harnesses information from a wide variety of related enzymes to inform cell biology and overcomes recurrent problems in the functional prediction of glycosyltransferase-related open-reading frames. Expand
Structure of a flavonoid glucosyltransferase reveals the basis for plant natural product modification
The three‐dimensional structure of VvGT1 has been determined, both in its ‘Michaelis’ complex with a UDP‐glucose‐derived donor and the acceptor kaempferol and in complex with UDP and quercetin, providing the foundation for understanding the mechanism of these enzymes in small molecule homeostasis. Expand
Insights into the oxidative degradation of cellulose by a copper metalloenzyme that exploits biomass components
It is demonstrated that copper is needed for GH61 maximal activity and that the formation of cellodextrin and oxidized cellodesxtrin products by GH61 is enhanced in the presence of small molecule redox-active cofactors such as ascorbate and gallate. Expand
A potent mechanism-inspired O-GlcNAcase inhibitor that blocks phosphorylation of tau in vivo.
Thiamet-G will find wide use in probing the functional role of O-GlcNAc in vertebrate brain, and it may also offer a route to blocking pathological hyperphosphorylation of tau in AD. Expand
Crystal structure of an N-terminal fragment of the DNA gyrase B protein
The crystal structure of an N-terminal fragment of the Escherichia coli DNA gyrase B protein, com-plexed with a nonhn/drolysable ATP analogue, has been solved at 2.5 Å resolution. It consists of twoExpand
Crystal structure of levansucrase from the Gram-negative bacterium Gluconacetobacter diazotrophicus.
A comparison of both structures, the mutagenesis data and the analysis of GH68 family multiple sequences alignment show a strong conservation of the sucrose hydrolytic machinery among levansucrases and also a structural equivalence of the Bs levanucrase Ca2+-binding site to the LsdA Cys339-Cys395 disulphide bridge, suggesting similar fold-stabilizing roles. Expand