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Biochemical Properties of Bovine Granulocytes
Summary Granulocyte fractions, containing an average of about 92% neutrophils, were isolated from bovine blood. The electron microscope observation of these fractions showed that neutrophil granulesExpand
The TAB1-p38α complex aggravates myocardial injury and can be targeted by small molecules
The data reveal that it is possible to selectively inhibit signaling downstream of p38α to attenuate ischemic injury and conclude that the interaction between the 2 proteins can be targeted with small molecules. Expand
Identification of I137M and Other Mutations That Modulate Incubation Periods for Two Human Prion Strains
It is argued that changing the surface charge in this region of PrP greatly altered the interaction between PrP isoforms during prion replication, and that strain-specified replication of prions is modulated by PrP sequence-specific interactions between the prion precursor PrPC and the infectious product PrPSc. Expand
New therapeutic targets in cardiology: p38 alpha mitogen-activated protein kinase for ischemic heart disease.
This review will focus on the ways in which p38 can be manipulated based on its mechanisms of activation and structure and how this knowledge has led to current cardiovascular clinical trials. Expand
The structure of Lethocerus troponin C: insights into the mechanism of stretch activation in muscles.
Using both synthetic peptides and recombinant proteins, it is shown that the N lobe of F1 TnC is not engaged in interactions with TnI, excluding a regulatory role of this domain, providing insights into mechanically stimulated muscle contraction. Expand
A comparative study of the biochemical properties of myeloperoxidase and eosinophil peroxidase.
Myeloperoxidase, the peroxidase of neutrophilic leukocytes, is genetically distinct from the eosinophil peroxidase as suggested by the absence of myeloperoxidase activity from neutrophils, but notExpand
Secretion of granule enzymes from alveolar macrophages. Regulation by intracellular Ca2+-buffering capacity.
If the energy supply is decreased by treating the macrophages with mitochondrial inhibitors such as oligomycin, cyanide, or rotenone and antimycin, Ca2+-dependent secretion is potentiated several fold and selective release of granule enzymes from macrophage exposed to A23187 is also stimulated by cytochalasin B (CB). Expand
Plasma membrane and phagosome localisation of the activated NADPH oxidase in elicited peritoneal macrophages of the guinea‐pig
The subecelluar distribution of the NADPH oxidase of guinea‐pig peritoneal‐elicited macrophages was investigated and was found to be enriched at the interface between 20 and 34 per cent of sucrose. Expand
Solution Structure of the Apo C-Terminal Domain of the Lethocerus F1 Troponin C Isoform
The results provide the first example of a TnC domain in which the presence of only one calcium ion is sufficient to induce a closed-to-open transition and clarify the role of calcium in stretch activation. Expand
Mining the PDB for tractable cases where X-ray crystallography combined with fragment screens can be used to systematically design protein-protein inhibitors. Two test cases illustrated by IL1β-IL1R
A bioinformatics analysis of the Protein Data Bank protein complexes revealed over 400 cases where the crystal lattice of the target in the free form is such that large portions of the interacting surfaces are free from lattice contacts and therefore accessible to fragments during soaks. Expand