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Structural analysis of a eukaryotic sliding DNA clamp–clamp loader complex
Sliding clamps are ring-shaped proteins that encircle DNA and confer high processivity on DNA polymerases. Here we report the crystal structure of the five-protein clamp loader complex (replicationExpand
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The chromodomains of the Chd1 chromatin remodeler regulate DNA access to the ATPase motor.
Chromatin remodelers are ATP-driven machines that assemble, slide, and remove nucleosomes from DNA, but how the ATPase motors of remodelers are regulated is poorly understood. Here we show that theExpand
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Post-Translational Modifications of Histones That Influence Nucleosome Dynamics
Nucleosomes are efficient DNA-packaging units. The fundamental protein unit of the nucleosome is the histone dimer, a simple α-helical domain possessing a highly basic, curved surface that closelyExpand
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The Chd1 chromatin remodeler shifts hexasomes unidirectionally
Despite their canonical two-fold symmetry, nucleosomes in biological contexts are often asymmetric: functionalized with post-translational modifications (PTMs), substituted with histone variants, andExpand
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Extranucleosomal DNA Binding Directs Nucleosome Sliding by Chd1
ABSTRACT Chd1- and ISWI-type chromatin remodelers can sense extranucleosomal DNA and preferentially shift nucleosomes toward longer stretches of available DNA. The DNA-binding domains of theseExpand
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Interdomain Communication of the Chd1 Chromatin Remodeler across the DNA Gyres of the Nucleosome.
Chromatin remodelers use a helicase-like ATPase motor to reposition and reorganize nucleosomes along genomic DNA. Yet, how the ATPase motor communicates with other remodeler domains in the context ofExpand
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Out-of-plane motions in open sliding clamps: molecular dynamics simulations of eukaryotic and archaeal proliferating cell nuclear antigen.
Sliding clamps are ring-like multimeric proteins that encircle duplex DNA and serve as mobile DNA-bound platforms that are essential for efficient DNA replication and repair. Sliding clamps areExpand
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Analysis of the role of PCNA-DNA contacts during clamp loading
BackgroundSliding clamps, such as Proliferating Cell Nuclear Antigen (PCNA) in eukaryotes, are ring-shaped protein complexes that encircle DNA and enable highly processive DNA replication by servingExpand
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The Replication Factor C Clamp Loader Requires Arginine Finger Sensors to Drive DNA Binding and Proliferating Cell Nuclear Antigen Loading*♦
Replication factor C (RFC) is an AAA+ heteropentamer that couples the energy of ATP binding and hydrolysis to the loading of the DNA polymerase processivity clamp, proliferating cell nuclear antigenExpand
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The Chd1 Chromatin Remodeler Shifts Nucleosomal DNA Bidirectionally as a Monomer.
Chromatin remodelers catalyze dynamic packaging of the genome by carrying out nucleosome assembly/disassembly, histone exchange, and nucleosome repositioning. Remodeling results in evenly spacedExpand
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