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Dimerization of Bovine F1-ATPase by Binding the Inhibitor Protein, IF1 *
TLDR
In mitochondria, the hydrolytic activity of ATP synthase is regulated by a natural inhibitor protein, IF1, and it is not known whether IF1 can bring about the dimerization of the F1F0-ATPase complex.
The synthesis of oligoribonucleotides containing O6-methylguanosine: the role of conserved guanosine residues in hammerhead ribozyme cleavage.
TLDR
The results show that the three conserved G residues play crucial but different role sin hammerhead cleavage.
Cloning, sequencing and expression of the gene for flavodoxin from Megasphaera elsdenii and the effects of removing the protein negative charge that is closest to N(1) of the bound FMN.
TLDR
Results suggest that glutamate 60 plays a major role in control of the redox properties of M. elsdenii flavodoxin, and they provide experimental support to an earlier proposal that the carboxylate on its side-chain is associated with a redox-linked pKa of 5.8 in the hydroquinone.
Oxidative coupling of furans and naphthoquinones: a potential route to anthracyclinones
2-Furyl-1,4-naphthoquinones have been prepared by treatment of mixtures of furans and naphthoquinones with chloranil and other oxidants, and used to synthesize quinizarin and
Cloning of electron-transferring flavoprotein from Megasphaera elsdenii.
TLDR
Electron-transferring flavoproteins are heterodimeric enzymes that contain FAD and which transfer reducing equivalents between other flavoenzymes that have similar functions in microorganisms such as Paracoccus denitrificans, Methylotroph WA3 1 and the strictly anaerobic rumen bacterium Megasphaera elsdenii.
ACE2, an activator of yeast metallothionein expression which is homologous to SWI5
TLDR
This study has isolated a gene, designated ACE2, which when present on a high-copy-number plasmid suppresses the copper-sensitive phenotype of an ace1-deletion strain and indicates that ACE2 plays a role in regulating basal-level expression of CUP1.
Analytical Centrifugation: Looking at Aggregation in Free Solution
An increasingly common question in structural biology now is the state of aggregation of a protein or complex in solution. In other words, is it monomeric, or has it aggregated to form some oligomer
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