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LysM, a widely distributed protein motif for binding to (peptido)glycans
Bacteria retain certain proteins at their cell envelopes by attaching them in a non‐covalent manner to peptidoglycan, using specific protein domains, such as the prominent LysM (Lysin Motif) domain.Expand
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A system to generate chromosomal mutations in Lactococcus lactis which allows fast analysis of targeted genes.
A system for generating chromosomal insertions in lactococci is described. It is based on the conditional replication of lactococcal pWV01-derived Ori+ RepA- vector pORI19, containing lacZ alpha andExpand
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Molecular cloning and nucleotide sequence of the gene encoding the major peptidoglycan hydrolase of Lactococcus lactis, a muramidase needed for cell separation.
A gene of Lactococcus lactis subsp. cremoris MG1363 encoding a peptidoglycan hydrolase was identified in a genomic library of the strain in pUC19 by screening Escherichia coli transformants for cellExpand
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Cell Wall Attachment of a Widely Distributed Peptidoglycan Binding Domain Is Hindered by Cell Wall Constituents*
The C-terminal region (cA) of the major autolysin AcmA of Lactococcus lactis contains three highly similar repeated regions of 45 amino acid residues (LysM domains), which are separated byExpand
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Genetic and biochemical characterization of the oligopeptide transport system of Lactococcus lactis.
The nucleotide sequence of a chromosomal DNA fragment of Lactococcus lactis subsp. lactis SSL135, previously implicated in peptide utilization, has been determined. The genes oppDFBCA, encoding theExpand
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Time-resolved determination of the CcpA regulon of Lactococcus lactis subsp. cremoris MG1363.
Carbon catabolite control protein A (CcpA) is the main regulator involved in carbon catabolite repression in gram-positive bacteria. Time series gene expression analyses of Lactococcus lactis MG1363Expand
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Novel Surface Display System for Proteins on Non-Genetically Modified Gram-Positive Bacteria
ABSTRACT A novel display system is described that allows highly efficient immobilization of heterologous proteins on bacterial surfaces in applications for which the use of genetically modifiedExpand
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AcmA of Lactococcus lactis is an N‐acetylglucosaminidase with an optimal number of LysM domains for proper functioning
AcmA, the major autolysin of Lactococcus lactis MG1363 is a modular protein consisting of an N‐terminal active site domain and a C‐terminal peptidoglycan‐binding domain. The active site domain isExpand
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Probing direct interactions between CodY and the oppD promoter of Lactococcus lactis.
CodY of Lactococcus lactis MG1363 is a transcriptional regulator that represses the expression of several genes encoding proteins of the proteolytic system. These genes include pepN, pepC, opp-pepO1,Expand
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ArgR and AhrC are both required for regulation of arginine metabolism in Lactococcus lactis.
The DNA binding proteins ArgR and AhrC are essential for regulation of arginine metabolism in Escherichia coli and Bacillus subtilis, respectively. A unique property of these regulators is that theyExpand
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