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A continuous spectrophotometric method for measuring the activity of serum alkaline phosphatase.
A continuous spectrophotometric method for measuring serum alkaline phosphatase activity is described. The effects of temperature, pH, substrate concentration, type and molarity of the buffer, sampleExpand
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Study of optimum buffer conditions for measuring alkaline phosphatase activity in human serum.
We have compared 23 compounds, with and without transphosphorylating properties, as buffer systems for human serum alkaline phosphatase activity, with p -nitrophenylphosphate as substrate. RelativeExpand
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Measurement of total alkaline phosphatase activity in human serum.
A one step Charcoal treated Alkaline phosphatase (ALP) reagent has been developed without affecting its basic ingredients. Conventionally, ALP measurement is based on the detection of phenol producedExpand
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Determination of the molar absorptivity of NADH.
The molar absorptivity of NADH at 340 nm has been determined by an indirect procedure in which high-purity glucose is phosphorylated by ATP in the presence of hexokinase, coupled to oxidation of theExpand
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Macro creatine kinase types 1 and 2: clinical significance in neonates and children as compared with adults.
We examined the case histories of seven pediatric patients (ages four days to 12 years) whose sera showed significant activities of "macro" creatine kinase (CK; EC two cases of macro CKExpand
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Optimum reaction conditions for human lactate dehydrogenase isoenzymes as they affect total lactate dehydrogenase activity.
Optimum reaction conditions at 30° ± 0.5° for two continuous spectrophotometric assay procedures, lactate to pyruvate (L → P) and pyruvate to lactate (P → L), were determined with respect to pH atExpand
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Free calcium in serum. I. Determination with the ion-specific electrode, and factors affecting the results.
Free (ionic) calcium in human serum was measured with a commercially available ion-specific electrode, together with a saturated-KCl salt bridge, a saturated-calomel reference electrode, and aExpand
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A referee method for the determination of total calcium in serum.
The first referee method of analysis (a method of known accuracy) in clinical chemistry has been developed through the cooperation of scientists from the National Bureau of Standards and eightExpand
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