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A continuous spectrophotometric method for measuring the activity of serum alkaline phosphatase.
A continuous spectrophotometric method for measuring serum alkaline phosphatase activity is described. The effects of temperature, pH, substrate concentration, type and molarity of the buffer, sample… Expand
Clinical Utilization of Alkaline Phosphatase Measurements
Study of optimum buffer conditions for measuring alkaline phosphatase activity in human serum.
We have compared 23 compounds, with and without transphosphorylating properties, as buffer systems for human serum alkaline phosphatase activity, with p -nitrophenylphosphate as substrate. Relative… Expand
Measurement of total alkaline phosphatase activity in human serum.
A one step Charcoal treated Alkaline phosphatase (ALP) reagent has been developed without affecting its basic ingredients. Conventionally, ALP measurement is based on the detection of phenol produced… Expand
Provisional recommendations on IFCC methods for the measurement of catalytic concentrations of enzymes. Part 2. IFCC method for aspartate aminotransferase.
Determination of the molar absorptivity of NADH.
- R. Mccomb, L. Bond, R. Burnett, R. C. Keech, G. Bowers
- Chemistry, Medicine
- Clinical chemistry
- 1 February 1976
The molar absorptivity of NADH at 340 nm has been determined by an indirect procedure in which high-purity glucose is phosphorylated by ATP in the presence of hexokinase, coupled to oxidation of the… Expand
Macro creatine kinase types 1 and 2: clinical significance in neonates and children as compared with adults.
We examined the case histories of seven pediatric patients (ages four days to 12 years) whose sera showed significant activities of "macro" creatine kinase (CK; EC 22.214.171.124): two cases of macro CK… Expand
Optimum reaction conditions for human lactate dehydrogenase isoenzymes as they affect total lactate dehydrogenase activity.
Optimum reaction conditions at 30° ± 0.5° for two continuous spectrophotometric assay procedures, lactate to pyruvate (L → P) and pyruvate to lactate (P → L), were determined with respect to pH at… Expand
Free calcium in serum. I. Determination with the ion-specific electrode, and factors affecting the results.
Free (ionic) calcium in human serum was measured with a commercially available ion-specific electrode, together with a saturated-KCl salt bridge, a saturated-calomel reference electrode, and a… Expand
A referee method for the determination of total calcium in serum.
The first referee method of analysis (a method of known accuracy) in clinical chemistry has been developed through the cooperation of scientists from the National Bureau of Standards and eight… Expand