Determination of clenbuterol from pork samples using surface molecularly imprinted polymers as the selective sorbents for microextraction in packed syringe.
- Wei Du, Chunmei Lei, Chunran Chang
- ChemistryJournal of Pharmaceutical and Biomedical Analysis
- 25 March 2014
Polyamine-like immunoreactivity in rat neurons
- K. Fujiwara, G. Bai, T. Kitagawa
- BiologyBrain Research
- 29 August 1997
Specificities of five kinds of antisera produced against crude drugs, pinella tuber, hoelen, glycyrrhizae radix, trichosanthes root and panax ginseng.
- T. Kitagawa, G. Bai, K. Fujiwara
- Biology, ChemistryBiological and Pharmaceutical Bulletin
- 15 March 1996
Five kinds of rabbit antisera produced againstfive kinds of crude drugs, Pinella Tuber, Glycyrrhizae Radix (GR), Trichosanthes Root, Hoelen, and Panax Ginseng, and five kinds of extracts of the same crude drugs were prepared, finding characteristic antigen specific to the corresponding antiserum.
Determination of thiocyanate metabolite of sodium nitroprusside in serum by spectrophotometry.
A sensitive, reproducible and rapid method for the spectrophotometric determination of thiocyanate, the metabolite of sodium nitroprusside, was developed. Acetonitrile was used to remove protein in…
Immunoelectron Microscopic Study for Polyamines
- K. Fujiwara, G. Bai, T. Kitagawa, D. Tsuru
- BiologyJournal of Histochemistry and Cytochemistry
- 1 November 1998
It is demonstrated that PA are predominantly located on free and attached ribosomes of the rough endoplasmic reticulum in the neurons of the lateral reticular nucleus of rat medulla oblongata, suggesting that PAs are one of the components of biologically active ribosome, being closely involved in the translation processes of protein biosynthesis.
Immunocytochemical Localization of Histamine in Enterochromaffin-like (ECL) Cells in Rat Oxyntic Mucosa: A Transmission Electron Microscopy Study Using Monoclonal Antibodies and Conventional…
- K. Fujiwara, G. Bai, C. Tamura, D. Tsuru
- BiologyJournal of Histochemistry and Cytochemistry
- 1 August 1999
It is suggested that in ECL cells HA is enzymatically synthesized in the cytoplasm, then is transported and stored in the cores of the granules before its release from the basal lamina.
Determination of urinary acetylpolyamines by a monoclonal antibody-based enzyme-linked immunosorbent assay (ELISA).
- K. Fujiwara, H. Kanetake, T. Kitagawa
- BiologyJournal of Biochemistry (Tokyo)
- 1 December 1995
The ASPM-2 ELISA for N1-Ac-Spd, which is the PA most relevant to the analysis of human urine among the three Ac-PAs mentioned above, is specific and accurate, and can easily be used to analyze large numbers of specimens in parallel.
Development and application of a sandwich enzyme immunoassay for Glycyrrhizae Radix protein (GRP) using monoclonal antibodies.
- G. Bai, K. Fujiwara, H. Tanimori, T. Kitagawa
- BiologyBiological and Pharmaceutical Bulletin
- 15 December 1997
The present SEIA was about 10-times more sensitive in detecting GRP than the selected antibody enzyme immunoassay for GRP previously developed using an antiserum to GR itself, and may provide a general analytical method for the quality control of GR-based TCMs.
A general enzyme immunoassay for the licorice root component contained in traditional Chinese medicines.
- T. Kitagawa, Z. Yang, S. Kondo
- BiologyBiological and Pharmaceutical Bulletin
- 15 March 1997
The SAEIA was successful in the detection and quantitative measurement of GR component contents in Chinese traditional medicines and the technique of selected antibody enzyme immunoassay (SAEIA) was applied to develop a sensitive general assay method.
Enzyme immunoassay for a characteristic protein in the animal crude drug lumbricus.
- G. Bai, K. Fujiwara, Y. Kaminishi, H. Z. Li, T. Kitagawa
- BiologyBiological and Pharmaceutical Bulletin
- 15 March 1998
The SAEIA for 70 kDa protein was simple, accurate, reproducible and may provide a general analytical method for the quality control of Lumbricus-based TCMs.
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