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Determination of clenbuterol from pork samples using surface molecularly imprinted polymers as the selective sorbents for microextraction in packed syringe.
TLDR
Under the optimized conditions, a simple and rapid method for the determination of CLB from pork samples was established by coupling with high performance liquid chromatography (HPLC) and it can be accomplished with 2min. Expand
Polyamine-like immunoreactivity in rat neurons
TLDR
The results may suggest that in neurons PAs are not transported axonally, but are located in conjunction with Nissl bodies (the rough endoplasmic reticulum), specified as sites for protein synthesis within cells. Expand
Specificities of five kinds of antisera produced against crude drugs, pinella tuber, hoelen, glycyrrhizae radix, trichosanthes root and panax ginseng.
TLDR
Five kinds of rabbit antisera produced againstfive kinds of crude drugs, Pinella Tuber, Glycyrrhizae Radix (GR), Trichosanthes Root, Hoelen, and Panax Ginseng, and five kinds of extracts of the same crude drugs were prepared, finding characteristic antigen specific to the corresponding antiserum. Expand
Determination of thiocyanate metabolite of sodium nitroprusside in serum by spectrophotometry.
A sensitive, reproducible and rapid method for the spectrophotometric determination of thiocyanate, the metabolite of sodium nitroprusside, was developed. Acetonitrile was used to remove protein inExpand
Immunoelectron Microscopic Study for Polyamines
TLDR
It is demonstrated that PA are predominantly located on free and attached ribosomes of the rough endoplasmic reticulum in the neurons of the lateral reticular nucleus of rat medulla oblongata, suggesting that PAs are one of the components of biologically active ribosome, being closely involved in the translation processes of protein biosynthesis. Expand
Immunocytochemical Localization of Histamine in Enterochromaffin-like (ECL) Cells in Rat Oxyntic Mucosa: A Transmission Electron Microscopy Study Using Monoclonal Antibodies and Conventional
TLDR
It is suggested that in ECL cells HA is enzymatically synthesized in the cytoplasm, then is transported and stored in the cores of the granules before its release from the basal lamina. Expand
Determination of urinary acetylpolyamines by a monoclonal antibody-based enzyme-linked immunosorbent assay (ELISA).
TLDR
The ASPM-2 ELISA for N1-Ac-Spd, which is the PA most relevant to the analysis of human urine among the three Ac-PAs mentioned above, is specific and accurate, and can easily be used to analyze large numbers of specimens in parallel. Expand
Development and application of a sandwich enzyme immunoassay for Glycyrrhizae Radix protein (GRP) using monoclonal antibodies.
TLDR
The present SEIA was about 10-times more sensitive in detecting GRP than the selected antibody enzyme immunoassay for GRP previously developed using an antiserum to GR itself, and may provide a general analytical method for the quality control of GR-based TCMs. Expand
Enzyme immunoassay for a characteristic protein in the animal crude drug lumbricus.
TLDR
The SAEIA for 70 kDa protein was simple, accurate, reproducible and may provide a general analytical method for the quality control of Lumbricus-based TCMs. Expand
Application of a licorice root specific protein to a general method for the assay of licorice root components in traditional Chinese medicines.
TLDR
The fuzzy SAEIA method was successfully applied for the detection and quantitative analysis of the GR component contained in traditional Chinese medicines. Expand
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