G. Backliwal

Publications15
h-index6
Citations628
Highly Influential Citations50
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Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions
TLDR
This protocol was reproducibly scaled-up to a working volume of 2 l, thus delivering >1 g of purified protein just 2 weeks after transfection, which is the fastest approach to gram quantities of protein ever reported from cultivated mammalian cells.
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High-density transfection with HEK-293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI.
TLDR
Successful in situ transfection of suspension-adapted HEK-293 cells with 25-kDa linear polyethyleneimine at densities up to 20 x 10(6) cells/mL in complex media followed by production at lower cell densities is reported.
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Valproic acid: a viable alternative to sodium butyrate for enhancing protein expression in mammalian cell cultures.
TLDR
With valproic acid (VPA), volumetric and specific productivities of 200 mg/L and 20 pg/cell/day, respectively, were achieved in HEK293E cells with a 10-day process and are recommended as a cost-effective alternative to this widely used enhancer of recombinant protein production from mammalian cells.
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Coexpression of acidic fibroblast growth factor enhances specific productivity and antibody titers in transiently transfected HEK293 cells.
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Polyethylenimine-based quality control assay for plasmid DNA.
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Disassembly of polyethylenimine-DNA particles in vitro: implications for polyethylenimine-mediated DNA delivery.
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High Cell Density Transient Gene Expression in HEK 293 EBNA Cells
TLDR
Results obtained by optimization of a PEI-mediated high-density transient transfection protocol in HEK-293E cells with emphasis on reducing the cost of DNA needed for transfections are presented.
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A Transient Gene Expression Process with Recombinant Antibody Titer of 0.5 g/L in CHO Cells
TLDR
An optimized transfection method for Chinese hamster ovary (CHO) cells is described that allows the transient production of recombinant antibody at titers approaching 0.5 g/L by two weeks post-transfection.
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Release of Plasmid-DNC from PEI/DNA Particles
TLDR
The results of these in vitro experiments are important for understanding the transport and release of plasmid DNA from PEI/DNA particles after transfection of cells.
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Comparison of lovastatin synthesis using glucose and lactose by metabolic flux analysis
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