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Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions
- G. Backliwal, M. Hildinger, Sébastien Chenuet, S. Wulhfard, M. de Jesus, F. Wurm
- BiologyNucleic acids research
- 10 July 2008
TLDR
High-density transfection with HEK-293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI.
- G. Backliwal, M. Hildinger, V. Hasija, F. Wurm
- BiologyBiotechnology and bioengineering
- 15 February 2008
TLDR
Valproic acid: a viable alternative to sodium butyrate for enhancing protein expression in mammalian cell cultures.
- G. Backliwal, M. Hildinger, Ivan Kuettel, Fanny Delegrange, D. Hacker, F. Wurm
- BiologyBiotechnology and bioengineering
- 1 September 2008
TLDR
Coexpression of acidic fibroblast growth factor enhances specific productivity and antibody titers in transiently transfected HEK293 cells.
- G. Backliwal, M. Hildinger, Sébastien Chenuet, M. Dejesus, F. Wurm
- Biology, EngineeringNew biotechnology
- 1 October 2008
Polyethylenimine-based quality control assay for plasmid DNA.
- M. Bertschinger, Cédric Burki, G. Backliwal, D. Hacker, M. Jordan, F. Wurm
- Biology, ChemistryAnalytical biochemistry
- 15 September 2006
Disassembly of polyethylenimine-DNA particles in vitro: implications for polyethylenimine-mediated DNA delivery.
- M. Bertschinger, G. Backliwal, Arnaud Schertenleib, M. Jordan, D. Hacker, F. Wurm
- Biology, ChemistryJournal of controlled release : official journal…
- 10 November 2006
High Cell Density Transient Gene Expression in HEK 293 EBNA Cells
- Divor Kiseljak, G. Backliwal, D. Hacker, L. Baldi, F. Wurm
- Biology
- 2012
TLDR
A Transient Gene Expression Process with Recombinant Antibody Titer of 0.5 g/L in CHO Cells
- Yashas Rajendra, G. Backliwal, M. Hildinger, S. Wulhfard, M. D. Jesus, F. Wurm
- Biology, Engineering
- 2012
TLDR
Release of Plasmid-DNC from PEI/DNA Particles
- M. Bertschinger, Arnaud Schertenleib, G. Backliwal, M. Jordan, F. Wurm
- Biology, Chemistry
- 2007
TLDR
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