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Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions
Transient transfection allows for fast production of recombinant proteins. However, the current bottlenecks in transient transfection are low titers and low specific productivity compared to stableExpand
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High-density transfection with HEK-293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI.
Recombinant proteins are of great commercial and scientific interest. Yet, most production methods in mammalian cells involve the time- and labor-consuming step of creating stable cell lines.Expand
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Valproic acid: a viable alternative to sodium butyrate for enhancing protein expression in mammalian cell cultures.
Various DNA methyl transferase inhibitors (iDNMTs) and histone deacetylase inhibitors (iHDACs) were screened for their ability to enhance transient gene expression (TGE) in Human Embryonic KidneyExpand
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Coexpression of acidic fibroblast growth factor enhances specific productivity and antibody titers in transiently transfected HEK293 cells.
Recombinant proteins are of great commercial and scientific interest. However, most current production methods using mammalian cells involve the time- and labor-intensive step of creating stable cellExpand
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Polyethylenimine-based quality control assay for plasmid DNA.
Keywords: Animals ; CHO Cells ; Cricetinae ; Cricetulus ; DNA/*analysis/chemistry/genetics ; Nanoparticles/analysis/chemistry ; Oligodeoxyribonucleotides/analysis/chemistry ;Expand
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Disassembly of polyethylenimine-DNA particles in vitro: implications for polyethylenimine-mediated DNA delivery.
Here a simple in vitro assay was used to investigate the disassembly of nanoparticles of polyethylenimine (PEI) and DNA. Particles were formed with various PEIs, allowed to mature for 10 min, andExpand
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High Cell Density Transient Gene Expression in HEK 293 EBNA Cells
Transient gene expression (TGE) is a simple method for the production of recombinant proteins in a few days. In this report we present results obtained by optimization of a PEI-mediated high-densityExpand
A Transient Gene Expression Process with Recombinant Antibody Titer of 0.5 g/L in CHO Cells
Here we describe our work towards an optimized transfection method for Chinese hamster ovary (CHO) cells that allows the transient production of recombinant antibody at titers approaching 0.5 g/L byExpand
Release of Plasmid-DNC from PEI/DNA Particles
The cationic polymer polyethylenimine (PEI) spontaneously forms complexes with DNA that efficiently transfect mammalian cells. To study the reversibility of the DNA/PEI interaction in vitro,Expand