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Engineering the internal cavity of neuroglobin demonstrates the role of the haem-sliding mechanism.
Point mutants of the neuroglobin internal cavity have been engineered and their functional and structural characterization shows that hindering the haem displacement leads to a decrease in CO affinity, whereas reducing the cavity volume without interfering with haem sliding has negligible functional effects. Expand
Structural features for the mechanism of antitumor action of a dimeric human pancreatic ribonuclease variant
The results suggest that the two hinge peptides and the two Leu28 side chains may provide an anchorage to a transient noncovalent dimer, which maintains Cys31 and Cys32 of the two subunits in proximity, thus stabilizing a quaternary structure, similar to that found for the non covalent swapped dimer of BSRNase, that allows the molecule to escape RI and/or to enhance the formation of the interchain disulfides. Expand
Redirecting P450 EryK specificity by rational site-directed mutagenesis.
Three single active-site mutants of EryK have been designed to allow hydroxylation of the nonphysiological substrate ErB, and the mutation of Met 86 to Ala to Ala is found to yield enzymatic activity on both ErB and ErD. Expand
Binding Properties of the Calcium-Activated F2 Isoform of Lethocerus Troponin C
The presence of two Ca2+ ions, one in each of the globular domains, increases the affinity for TnH by at least 1 order of magnitude, which could account for stretch activation in insect flight muscles. Expand
Determinants of neuroglobin plasticity highlighted by joint coarse-grained simulations and high pressure crystallography
The structural response to pressure of neuroglobin (Ngb), a hexacoordinate globin likely to be involved in neuroprotection, is detailed, showing that Ngb hinges around a rigid mechanical nucleus of five hydrophobic residues during its conformational transition induced by gaseous ligand. Expand
Ligand pathways in neuroglobin revealed by low-temperature photodissociation and docking experiments
Ultralow-temperature X-ray crystallography, in crystallo microspectroscopy and X-ray absorption spectroscopy were used to study the carbon monoxide photodissociation intermediate in neuroglobin.Expand