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IMMUNOASSAY OF BOVINE AND HUMAN PARATHYROID HORMONE.
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Use of polyethylene glycol to separate free and antibody-bound peptide hormones in radioimmunoassays.
ABSTRACT Aqueous polyethylene glycol causes precipitation of antibody-bound peptide hormones labeled with radioactive iodine with little or no precipitation of free hormones. Based on this finding, aExpand
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Preparation of viable isolated bovine parathyroid cells.
A method is described for the preparation of isolated bovine parathyroid cells. Fresh, minced bovine parathyroid tissue is incubated with 2 mg/ml collagenase and 50 mug/ml DNAse for 1 h at 37 C withExpand
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Pseudohypoparathyroidism: defective excretion of 3',5'-AMP in response to parathyroid hormone.
Urinary excretion of cyclic adenosine 3',5'-monophosphate (3',5'-AMP) was tested in normal subjects and patients with pseudohypoparathyroidism, idiopathic hypoparathyroidism, surgicalExpand
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Resistance to multiple hormones in patients with pseudohypoparathyroidism. Association with deficient activity of guanine nucleotide regulatory protein.
Abstract Pseudohypoparathyroidism type I is characterized by resistance (defined as a deficient urinary cAMP response) to parathyroid hormone and, in most cases, hypocalcemia and hyperphosphatemia.Expand
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Isolation of parathyroid hormone after extraction with phenol.
  • G. Aurbach
  • Biology, Medicine
  • The Journal of biological chemistry
  • 1 December 1959
In 1925, Collip (1) reported that an endocrine substance, extracted from parathyroid tissue with hot hydrochloric acid, corrected the hypocalcemia which followed parathyroidectomy. Extracts made byExpand
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The effect of parathyroid hormone on the concentration of adenosine 3',5'-monophosphate in skeletal tissue in vitro.
Abstract Adenosine 3',5'-monophosphate (3',5'-AMP) was assayed in calvaria isolated from fetal rats at term. A marked increase in 3',5'-AMP was detected in vitro within 1 min after adding purifiedExpand
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Synthesis of a biologically active N-terminal tetratriacontapeptide of parathyroid hormone.
Determination of the amino acid sequence of bovine parathyroid hormone has led to the synthesis of a tetratriacontapeptide corresponding to the amino-terminal 1-34 residues of the native molecule.Expand
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The amino acid sequence of bovine parathyroid hormone I.
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Recurrent hyperparathyroidism from an autotransplanted parathyroid adenoma.
AUTOTRANSPLANTATION of human parathyroid tissue into the forearm prevents hypoparathyroidism after total removal of parathyroid tissue from the neck.1 , 2 This procedure has been principally appliedExpand
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