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The RelA/SpoT Homolog (RSH) Superfamily: Distribution and Functional Evolution of ppGpp Synthetases and Hydrolases across the Tree of Life
RelA/SpoT Homologue (RSH) proteins, named for their sequence similarity to the RelA and SpoT enzymes of Escherichia coli, comprise a superfamily of enzymes that synthesize and/or hydrolyze theExpand
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Recent functional insights into the role of (p)ppGpp in bacterial physiology
The alarmones guanosine tetraphosphate and guanosine pentaphosphate (collectively referred to as (p)ppGpp) are involved in regulating growth and several different stress responses in bacteria. InExpand
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Distinct XPPX sequence motifs induce ribosome stalling, which is rescued by the translation elongation factor EF-P
Significance During protein synthesis, ribosomes catalyze peptide-bond formation between amino acids with differing efficiency. We show that two or more consecutive prolines induce ribosome stalling,Expand
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Evolution of nonstop, no-go and nonsense-mediated mRNA decay and their termination factor-derived components
BackgroundMembers of the eukaryote/archaea specific eRF1 and eRF3 protein families have central roles in translation termination. They are also central to various mRNA surveillance mechanisms,Expand
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GTP-dependent structural rearrangement of the eRF1:eRF3 complex and eRF3 sequence motifs essential for PABP binding
Translation termination in eukaryotes is governed by the concerted action of eRF1 and eRF3 factors. eRF1 recognizes the stop codon in the A site of the ribosome and promotes nascent peptide chainExpand
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Lys34 of translation elongation factor EF-P is hydroxylated by YfcM.
Lys34 of the conserved translation elongation factor P (EF-P) is post-translationally lysinylated by YjeK and YjeA--a modification that is critical for bacterial virulence. Here we show that theExpand
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Mechanism of Tetracycline Resistance by Ribosomal Protection Protein Tet(O)
Tetracycline resistance protein Tet(O), which protects the bacterial ribosome from binding the antibiotic tetracycline, is a translational GTPase with significant similarity in both sequence andExpand
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Evolution of elongation factor G and the origins of mitochondrial and chloroplast forms.
Protein synthesis elongation factor G (EF-G) is an essential protein with central roles in both the elongation and ribosome recycling phases of protein synthesis. Although EF-G evolution is predictedExpand
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Mitochondrial translation initiation machinery: Conservation and diversification☆
The highly streamlined mitochondrial genome encodes almost exclusively a handful of transmembrane components of the respiratory chain complex. In order to ensure the correct assembly of theExpand
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Negative allosteric regulation of Enterococcus faecalis small alarmone synthetase RelQ by single-stranded RNA
Significance Bacteria regulate their intracellular environment through two ubiquitous posttranscriptional regulatory mechanisms: enzymatic synthesis of small signaling molecules that allostericallyExpand
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