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Intracellular Trafficking of Brucella abortus in J774 Macrophages
  • G. Arenas, Ana Sandra Staskevich, Alejandro Aballay, L. Mayorga
  • Biology, Medicine
  • Infection and Immunity
  • 1 July 2000
To monitor the intracellular transport of B. abortus in macrophages, the kinetics of fusion of phagosomes with preformed lysosomes labeled with colloidal gold particles was observed by electron microscopy, indicating that the bacteria alter phagosome maturation in Macrophages. Expand
Distribution of Botulinum Toxin-Producing Clostridia in Soils of Argentina
It is observed that the presence of botulinum toxin-producing clostridia in soil samples from five geographical regions of Argentina has a great multiplicity of serological types and a higher prevalence in nonvirgin soils than in virgin soils. Expand
Analysis of the rumen bacterial diversity of goats during shift from forage to concentrate diet.
Three different culture-independent methods used in this study show that high proportion of concentrate in goat diet does not induce any serious disturbance of their rumen ecosystem and indicate the good adaptive response of caprine ruminal bacteria to incorporation of corn into the diet. Expand
Isolation of Pseudobutyrivibrio ruminis and Pseudobutyrivibrio xylanivorans from rumen of Creole goats fed native forage diet
The identification of bacterial strains involved in the rumen fermentation helps to explain the ability of these animals to digest fiber plant cell wall contained in native forage species. Expand
A phorbol ester‐binding protein is required downstream of Rab5 in endosome fusion
An in vitro endosome fusion assay supplemented with recombinant Rab5 proteins suggests that the phorbol ester binding protein is acting downstream of Rab5 in endosomes fusion. Expand
[First outbreak of botulism caused by Clostridium botulinum subtype Af].
Quantitative neutralization tests showed that this toxin consists of a major type A antigenic component and a minor type F component, defining its identity as a subtype Af, described in Argentina fifteen years ago, and not detected until now in other parts of the world. Expand
Calcium- and zinc-binding proteins in intracellular transport.
Calcium binding proteins regulate several aspects of the vesicle fusion mechanism mediated by NSF (N-ethylmaleimide sensitive fusion factor), and at least in some regulated exocytosis, calcium-binding proteins are the trigger for fusion downstream of NSF. Expand
Brucella alters endocytic pathway in J774 macrophages
The results indicate that the virulent strain not only occupies two different kinds of compartments but also alters the endocytic pathway of the cell it parasitizes, unlike what has been reported for non-professional phagocytes, like HeLa cell. Expand
Identification of GH10 xylanases in strains 2 and Mz5 of Pseudobutyrivibrio xylanivorans
Identifying xylanase-encoding genes and activity of these enzymes are a step toward understanding possible functional role of P. xylanivorans in the rumen ecosystem and contribute to providing an improved choice of enzymes for improving fiber digestion in ruminant animals, agricultural biomass utilization for biofuel production, and other industries. Expand