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SUMMARY: The conditions for luxuriant axenic cultivation of the trypanosomid flagellate Crithidia fasciculata in chemically-defined media were determined. Improvements over the hitherto published media include higher concentrations of amino acids, folic acid (where this is the sole source of pteridine) and purines and the inclusion of threonine and Tween(More)
The rodent gap junction protein connexin32 (Cx32) is highly expressed in hepatocytes, less abundantly in Schwann cells and oligodendrocytes, and at low levels in the early mouse embryo. In both hepatocytes and Schwann cells, Cx32 expression is directed by alternative promoter regions (P1 and P2) which activate differently spliced transcript isoforms. Here(More)
N6-methyladenine (6-methylaminopurine [6-MA]), a plant growth regulator and a normal constituent of nucleic acids, has been found to inhibit the growth of Trypanosoma cruzi, Leishmania braziliensis, L. donovani, L. tarentolae, L. mexicana, and Crithidia fasciculata. The extent of growth inhibition in these organisms is related to the sensitivity of guanine(More)
Teleost fish experience passive osmotic water influx in fresh water (FW) and water outflux in salt water, which is normally compensated by water flow driven by active ion transport mechanisms. Euryhaline fish may also minimize osmotic energy demand by "behavioral osmoregulation", seeking a medium isotonic with their body fluids. Our goal was to evaluate the(More)
We have developed a flow-through method for measuring oxygen consumption in fish which allows continuous monitoring over periods of days with good accuracy. Our goal was to determine the changes in basal metabolic rate in estuarine fish as a function of salinity. We show that in Fundulus heteroclitus, the oxygen consumption drops by 50% during the first 12(More)
DOBSON, JAMES G., JR., AND GEORGE W. KIDDER III. Edye damage effect in in vitro frog skin preparations. Am. J. Physiol. 214 (4) : 719-724. 1968.-When frog skin is mounted between conventional chambers, some of the tissue in the exposed area is damaged by stresses imposed by the clamping process. It has been possible to measure the extent and characteristics(More)
Upon passing a step current through the frog gastric mucosa, a transient response in voltage is observed, which can formally be represented by several types of model systems, although some models require elements which are hard to visualize in terms of the known morphology of the mucosa. A physically reasonable model can be constructed by considering the(More)