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SUMMARY: The conditions for luxuriant axenic cultivation of the trypanosomid flagellate Crithidia fasciculata in chemically-defined media were determined. Improvements over the hitherto published media include higher concentrations of amino acids, folic acid (where this is the sole source of pteridine) and purines and the inclusion of threonine and Tween(More)
Teleost fish experience passive osmotic water influx in fresh water (FW) and water outflux in salt water, which is normally compensated by water flow driven by active ion transport mechanisms. Euryhaline fish may also minimize osmotic energy demand by "behavioral osmoregulation", seeking a medium isotonic with their body fluids. Our goal was to evaluate the(More)
The rodent gap junction protein connexin32 (Cx32) is highly expressed in hepatocytes, less abundantly in Schwann cells and oligodendrocytes, and at low levels in the early mouse embryo. In both hepatocytes and Schwann cells, Cx32 expression is directed by alternative promoter regions (P1 and P2) which activate differently spliced transcript isoforms. Here(More)
We have developed a flow-through method for measuring oxygen consumption in fish which allows continuous monitoring over periods of days with good accuracy. Our goal was to determine the changes in basal metabolic rate in estuarine fish as a function of salinity. We show that in Fundulus heteroclitus, the oxygen consumption drops by 50% during the first 12(More)
PURPOSE Treatment of de-epithelialized human corneas with riboflavin (RF) + long-wavelength ultraviolet light (UVA; RFUVA) increases corneal stroma tensile strength significantly. RFUVA treatment retards the progression of keratoconus, perhaps by cross-linking of collagen molecules, but exact molecular mechanisms remain unknown. Research described here(More)