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We studied the interaction of cis-diammine Pt(II)-bridged bis-netropsin, cis-diammine Pt(II)-bridged bis-distamycin and oligomethylene-bridged bis-netropsin with synthetic DNA fragments containing pseudosymmetrical AT-rich nucleotide sequences and compared it with the interaction of the parent compounds netropsin and distamycin A. For fragments containing(More)
Interaction of DNA with the analogs of the antibiotic distamycin A having different numbers of pyrrolcarboxamide groups and labeled with dansyl was studied. The binding isoterms of the analogs to synthetic polydeoxyribonucleotides were obtained. Analysis of the experimental data leads to the following conclusions: (1) the free energy of binding of the(More)
The design and DNA binding activity of beta-structure-forming peptides and netropsin-peptide conjugates are reported. It is found that a pair of peptides-S,S'-bis(Lys-Gly-Val-Cys-Val-NH-NH-Dns)-bridged by an S-S bond binds at least 10 times more strongly to poly(dG).poly(dC) than to poly(dA).poly(dT). This peptide can also discriminate between 5'-GpG-3' and(More)
The mouse mammary tumor virus (MMTV) promoter is induced by glucocorticoid hormone. A robust hormone- and receptor-dependent gene activation could be reproduced in Xenopus laevis oocytes. The homogeneous response in this system allowed a detailed analysis of the DNA-protein interactions following hormone activation. The strategy of artificial regulating of(More)
296 Currently, the population has not been vaccinated against variola virus; as a result, children and the majority of adult population younger than 27–30 years old are not immune to smallpox. In view of this, designing and synthesis of new compound that can effectively inhibit reproduction of orthopox viruses is a relevant problem of both theoretical and(More)
Equations are derived for description of cooperative binding of large ligands to a homogeneous polynucleotide lattice for a wide variety of binding models. Both short- and long-range interactions between nearest-neighbour bound ligands are taken into account. It is shown that cooperative binding of ligand at high levels of occupancy can be described with(More)
Currently, significant progress has been made in the design and synthesis of site-specific DNA-binding agents that are analogues of the natural pyrrolecarboxamide antibiotics netropsin and distamycin and the bis benzimidazole dye Hoechst 33258 [1–7]. These compounds bind in the minor DNA groove to runs of three to five consecutive AT-base pairs. All of them(More)
The design of compounds that can selectively bind to DNA regions with specified nucleotide sequence is a topical problem of molecular biology [1–5]. In this work, we propose a new approach to the synthesis of specific DNA-binding compounds, which is based on the use of a combination of pyrrole(imidazole)carboxamide and new pseudopeptide fragments consisting(More)