Learn More
The binding of long multisite ligands to double-stranded nucleic acids is considered. The ligand is taken as a lattice of AT- and GC-specific reaction centres the sequence of which is complementary to the base pair sequence in the specific ligand interaction site on DNA. Exact equations are derived for the two cases: that when a ligand binds in a fixed(More)
The DNA-binding and antiviral activitus of bis-netropsins in which two monomers are attached covalently via three glycin residue were studied. These compounds have the same C-end groups but contain clusters with different numbers of lysine residues at the N-end of the molecule. In the homologous series of these compounds, bis-neropsins containing 15 and 31(More)
The search for inhibitors of reproduction of DNAcontaining viruses among compounds interacting with viral DNA is developed insufficiently. The use of bislinked derivatives of netropsin and distamycin, synthetic DNA-binding ligands composed of two derivatives of netropsin or distamycin covalently bound to each other with various linkers, is a possible(More)
Spectrophotometric methods are used to study the binding to DNA of Actinomycin D (AMD) and its analogues: 7-nitro-AMD; 7-amino-AMD; 7-(Z-Val-Glo-NH)-AMD; 7-(AcO- . +H2-Val-Glo-NH)-AMD; 7-(AcO- . +H2-Val-Glo-Val-Glo-NH)-AMD. The binding constants are calculated from the binding isotherm of AMD and those of the AMD analogues to calf thymus DNA obtained by(More)
Experimental data are reported on DNA-cleaving activity of the synthetic netropsin analogs consisting of the two N-propylpyrrole carboxamide units linked covalently through two or three glycine residues to a copper-chelating tripeptide glycyl-glycyl-L-histidine. Incubation of DNA restriction fragment and netropsin analog in the presence of ascorbate,(More)
The binding to DNA of Pt-bis-Nt and its modified analogue (Pt*-bis-Nt), which differs from Pt-bis-Nt by the fact that the connecting chain between two netropsin fragments contains two additional glycine residues, has been studied. Elongating the chain in the bis-netropsin molecule increases the cytotoxicity and leads to a complete disappearance of the(More)
Design and synthesis of selective antiviral DNA-binding compounds and the analysis of the molecular mechanisms of their action pose an urgent problem for molecular biology and medicine. In this field, important results were obtained when new ligands were synthesized using the analogs of the pyrrol carboxyamide antibiotics—netropsin and distamycin—as(More)
79 Modern methods of treatment of human infections caused by herpes virus are based on the use of modified nucleosides (acyclovir (ACV), ganciclovir, etc.) as drugs. After triphosphorylation, these compounds serve as substrates of viral DNA polymerase. During replication of viral DNA, they incorporate into the growing polynucleotide chain and inhibit(More)
Data obtained show that antiviral activities of bis-linked netropsin derivatives are targeted by specific complexes formed by helicase UL9 of herpes simplex virus type 1 with viral DNA replication origins, represented by two OriS sites and one OriL site. According to the results of footprinting studies bis-netropsins get bound selectively to an A+T-cluster(More)