G. Taugner

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Phosphoryl group transfer from ATP to ADP occurred in the isolated membrane of catecholamine storage vesicles. The reaction was accelerated by extraction of the membranes with 50% (v/v) acetone and by treatment with 1% (v/v) Triton X-100. The phosphoryl group transfer reaction was activated by Mg2+ and Mn2+. The activation profile differed from that(More)
The reactions of adenosine 14C- and γ32P-labelled ATP with isolated membranes from catecholamine storage vesicles of the bovine adrenal medulla were studied. In presence of Mg2+ about twice as much of 32P-radioactivity combined with the membrane as 14C-adenosine compounds at 31°C and also at 0°C, while in the absence of Mg2+ the amounts of 14C and 32P(More)
The thiol groups of the vesicular protein of bovine adrenal medulla were allowed to react with the bifunctional thiol reagent bis-(N-maleimidomethyl) ether and with the monofunctional thiol reagent N-ethylmaleimide, and the ATP-dependent and -independent catecholamine fluxes of the modified preparations were studied. 1. During the initial phase of the(More)
The terminal phosphate group of ATP was transferred to ADP by an enzyme present in the soluble core proteins of adrenal medulla catecholamine storage vesicles. It was purified 10-30-fold by DEAE Sephadex chromatography (Fraction I). The enzyme required divalent metal ions for activation; Mn2+ was almost as effective as Mg2+, but Ca2+ was only a weak(More)
  • G. Taugner
  • Naunyn-Schmiedeberg's Archives of Pharmacology
  • 2004
1. The net uptake of noradrenaline occurring in the presence of ATP as well as the adenosine triphosphatase activity of purified membranes isolated from bovine adrenomedullary storage vesicles reach maximal values at pH 7.4. However, the steep pH-dependent enhancement of the enzymatic activity between pH 5.0 and 6.2 does not correspond to any catecholamine(More)
The kinetics of catecholamine-storage in the vesicles of the adrenal medulla is described. 1. Vesicle-bound catecholamine is released in the absence of ATP. This efflux of catecholamine is ten times larger than the influx, resulting in an elevated catecholamine level in the medium. 2. In the presence of ATP influx and efflux of catecholamine are balanced.(More)