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Preliminary taxonomic characterization of isolate L420-91 has revealed that this organism is closely related to the species Bacillus aneurinolyticus. The bacterium is covered by a squarely arranged crystalline surface layer composed of identical glycoprotein subunits with an apparent molecular mass in the range of 109 kDa. A total carbohydrate content of(More)
In large variants of adenylate kinase the AMP and ATP substrates are buried by a domain rotating by 90 degrees. Here conformational changes responsible for this domain closure are determined by an analysis of the open state of beef heart mitochondrial adenylate kinase and the closed state of Escherichia coli adenylate kinase. Although these two proteins(More)
The diacetamidodideoxymannuronic-acid-containing glycan of Bacillus stearothermophilus NRS 2004/3a with the repeating unit structure [-->4)-beta-D-ManpA2,3(NAc)2-(1-->6)-alpha-D-Glcp-(1-->4)-beta-D-+ ++ManpA2,3 (NAc)2-(1-->3)-alpha-D-GlcpNAc-(1-->], was examined to identify its linkage to the bacterial cell wall. In a previous paper it was suggested that(More)
If phase-measurement interferometry (PMI) is applied for a high numerical aperture (NA), specific errors can occur. The resulting phase no longer varies linearly with the height difference in the test surface. Thus, for height measurements, a constant correction factor or NA scaling factor, which depends only on the NA and the illumination of its stop, does(More)
The rotation method for the absolute testing of three flats by the evaluation of four interference patterns of pairs of these flats is developed further. Least-squares methods for determining and minimizing the effect of random measuring errors are fully applied. This application makes an optimal resolution in depth and an enhanced lateral resolution(More)
A further-developed three-plate rotation method [Appl. Opt. 31, 3767 (1992)] for the absolute testing of flats is used as a measuring method for establishing flatness standards. A special phase-stepping Fizeau interferometer was built that permits flats of diameters up to 200 mm to be tested. First results show a mean error between 0.002 and 0.003 λ in the(More)
Previously, the repeating-unit structure of the S-layer glycoprotein from the eubacterium Bacillus alvei CCM 2051 has been determined to be [-->3)-beta-D-Galp-(1-->4)-[alpha-D-Glcp-(1-->6)-]-beta-D-ManpNAc- (1-->]n (E. Altman, J.-R. Brisson, P. Messner, and U. B. Sleytr, Biochem. Cell Biol. 69:72-78, 1991). Nuclear magnetic resonance spectroscopic(More)
A series of derivatives of the novel cyclopeptolide 1 was prepared, and their ability to chemosensitize multi drug resistant CHO and KB cells in vitro was evaluated. In contrast to the parent compound, several of the derivatives were found to be highly active. In particular, conversion of the R-lactic acid residue of 1 into its S-isomer via lactone ring(More)
Allyl 2-acetamido-2-deoxy-3,4-O-(1,1,3,3-tetraisopropyldisiloxan-1,3- diyl)-beta-D- glucopyranoside was coupled with methyl (4,5,7,8-tetra-O-acetyl-3-deoxy-alpha-D-manno-2-octulopyranosyl bromide)onate (1) to give a good yield of the alpha-(2----6)-linked disaccharide, isolated after deacetylation and regioselective conversion into the corresponding(More)
Glycosylation of methyl (allyl 7,8-O-carbonyl-3-deoxy-alpha-D-manno-2-octulo-pyranosid)o nate with an alpha-(2----4) linked per-O-acetylated KDO-disaccharide bromide derivative under Helferich conditions afforded a 2:1 mixture of the alpha- and beta-linked trisaccharide derivatives in 50% yield. Removal of the protecting groups gave sodium O-[sodium(More)