Learn More
Human tissues, both biopsy and postmortem, and tissues from rodents were fixed by microwaves at various temperatures and compared against formaldehyde-fixed material. Conventional stains, including trichromes, worked well. Red cell were lysed, but white cells were fixed, thus permitting diagnoses of various inflammatory states. Malignant cells were equally(More)
Epidermal growth factor (EGF) has been implicated in mitogenesis and oncogenesis in the gastrointestinal tract. To determine the role of EGF in oesophageal disease, its quantity and distribution in the oesophageal mucosa of control subjects and patients with oesophageal disease were studied. Oesophageal biopsy specimens, taken 20-40 cm from the incisors in(More)
The quantity and distribution of epidermal growth factor receptors (EGF-R) in oesophageal mucosa was studied in the oesophagus in order to determine its role in oesophageal disease. Fifty five biopsies were taken from different levels of the oesophagus in 25 consecutive patients undergoing endoscopy. Another group of eight patients with histologically(More)
The effect of advanced old age on the nerve cell content of the cerebral cortex was examined in 19 non-demented persons aged 69-95 years, using a Quantimet 720 image analysing computer to make area proportion measurements. Neurone loss around 1.0% per annum was found both in the neocortex and in the medial hippocampus. There was also significant shrinkage(More)
Intradermal injection of purified protein derivative produced typical delayed type hypersensitivity reactions in five healthy human subjects. The major subpopulations of lymphocytes and certain accessory cells were located in frozen sections of biopsies of the lesions with monoclonal antibodies and immunohistochemical staining. The densities (expressed as(More)
A new simple modification to the silver staining of nucleolar organiser regions (AgNORs) was devised which, by performing the incubation with the slide inverted, results in minimal undesirable background staining, a persistent problem. Inverted incubation is facilitated by the use of a commercially available plastic coverplate. This technique has several(More)
Placentae from normal term pregnancies, from "normal" preterm pregnancies and from pregnancies complicated by pre-eclampsia have been analysed by computerized histometry. An immunoperoxidase technique was used to stain specifically human placental lactogen in the syncytiotrophoblast. The percentage and mass of syncytiotrophoblast were measured using an(More)
The immunoperoxidase staining of 84 primary invasive breast carcinomas with four monoclonal antibodies (BRST-1, HMFG1, EMA, B72.3) was evaluated by semiquantitative light microscopical examination and quantitative image analysis. Major differences in the staining of the tumours for each of the monoclonal antibodies was observed. Correlation between(More)