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DNA in prepared chromosomes from the larval salivary glands of Drosophila melanogaster was hybridized with [125I]-labeled 5S and tRNA from the same organism. Autoradiography revealed that radioactivity was frequently bound to all regions of the slides, masking labeling of the chromosomes. Acetylation of the preparations before hybridization prevented the(More)
Kinetin stimulated phosphorylation of protein in floated Chinese-cabbage leaf discs, but inhibited protein phosphorylation in nuclei+chloroplast extracts from Chinese-cabbage or tobacco leaves. Kinetin also inhibited protein phosphorylation in isolated tobacco nuclei or nuclei from carrot secondary-phloem tissue. Purified Chinese-cabbage leaf ribosomes(More)
The nucleotide sequence of Drosophila melanogaster lysine tRNA2 was determined to be: pG-C-C-C-G-G-C-U-A-m2G-C-U-C-A-G-D-C-G-G-D-A-G-A-G-C-A-psi-G-A-G-A-C-U-C-U-U-t6A-A-psi-C-U-C-A-G-G-m7G-D-C-G-U-G-G-G-Xm-U-C-G-m1A-G-C-C-C-C-A-C-G-U-U-G-G-G-C-G-C-C-A(OH). With minor differences in the state of modification of some nucleotides, the sequence is the same as(More)
Aging and disease processes may be due to deleterious and irreversible changes produced by free radical reactions. The enzyme copper-zinc superoxide dismutase (Cu-Zn SOD; superoxide:superoxide oxidoreductase, EC 1.15.1.1) performs a protective function by scavenging superoxide radicals. The Cu-Zn SOD gene (Sod) cloned from Drosophila melanogaster was(More)
The Cu-Zn superoxide dismutase (SOD) cDNA and genomic DNA from Drosophila melanogaster have been isolated. The sequence of the coding region for the Sod gene as well as 413 bp of the 5'-untranslated region, 247 bp of the 3'-flanking DNA, and the single 725-bp intron have been determined [Seto et al., Nucleic Acids Res. 15 (1987) 10601]. The sequence reveals(More)