G. L. Atkins

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Ascorbic acid is catabolized to yield oxalate from carbon atoms 1 and 2 (1, 2) and carbon dioxide from carbon atom 1 in man as well as in other species (3-5). It has also been shown that n-glucuronolactone is converted to ascorbic acid in the human 63. The present studies were designed to determine the n-ascorbic acid metabolic pool size and turnover rate,(More)
1. Experimental progress curves were simulated for a reaction obeying Michaelis-Menten kinetics. 2. K(m) and V were estimated (a) by fitting the integrated Michaelis-Menten equation to the progress curves, and (b) from the initial slopes of the curves (i.e. from initial velocities). 3. The integrated equation could not be fitted successfully by a non-linear(More)
The Michaelis-Menten equation was fitted to simulated data containing different sorts of error by using the three linear transformations, and the methods of S. R. Cohen [Anal. Biochem. (1968) 22, 549-552], R. Eisenthal & A. Cornish-Bowden [Biochem. J. (1974) 139, 715-120], F. de M. Merino [Biochem. J. 143, 93-95] and G. N. Wilkinson [Biochem. J. (1961) 808(More)
A number of algebraic expressions for the solute concentration for use with the Michaelis-Menten equation during the analysis of data from intestinal perfusion experiments have been investigated. It is concluded that the most suitable, especially if water absorption is occurring, is of the form: S = (S initial--S effluent)/ln(S initial/S effluent).
The lipocalin apolipoprotein D (ApoD) is associated in human plasma with lecithin-cholesterol acyl transferase. It has also been found in high concentration in the fluid of gross cystic disease of the mammary gland. Using protein fluorescence quenching, it is shown that ApoD binds arachidonic acid (Ka of 1.6 x 10(8) M-1) and as previously known progesterone(More)
1. Published data for absorption kinetics have been fitted by non-linear regression to (i) a single Michaelis-Menten function, (ii) a Michaelis-Menten function plus a linear term and (iii) a sum of two Michaelis-Menten functions. A series of criteria have been drawn up to establish the goodness of fit in each case. 2. In 17 out of 35 cases the(More)
The methods of Atkins & Nimmo (1973) and Fernley (1974) for fitting the integrated Michaelis-Menten equation were compared by using the same sets of simulated experimental data. The method of Fernley (1974) is to be preferred because it gives precise and unbiased estimates of the Michaelis-Menten parameters over a wide range of substrate concentrations.(More)