G Jurjen E Oosterhuis

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OBJECTIVE [1] To determine whether apoptosis can be measured in ejaculated spermatozoa by flow cytometry using the Annexin V assay, which measures expression of phosphatidylserine on the outer leaflet of the cell membrane, or the TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP [deoxy-uridine triphosphate] nick end labeling) assay, which measures(More)
Insulin-like growth factor-I (IGF-I) and IGF binding protein-3 (IGFBP-3) play an important role in regulating follicle growth and maturation. We have evaluated whether responsiveness to gonadotrophins during an in-vitro fertilization (IVF) treatment is related to follicular fluid IGF-I and IGFBP-3 concentrations. We also investigated if a difference is(More)
OBJECTIVE To summarize the evidence for the use of commonly accepted fertility tests in subfertile women with ovulation problems. DESIGN Systematic review. SETTING Not applicable. PATIENT(S) The study population included women starting with clomiphene citrate (CC) as first-line treatment, women starting with second-line treatment if CC failed to(More)
We investigated whether the hypo-osmotic swelling (HOS) test is a reliable screening method for routine semen specimen quality screening. Of 159 male patients, 171 semen samples were investigated using the HOS test as well as routine semen specimen screening used in our clinic. There was a significant correlation between the HOS test and most semen(More)
BACKGROUND A method was previously described to measure FSH reliably in unextracted urine. The aim of the current study was to establish the course of FSH measured in urine throughout the cycle. METHOD Daily urinary FSH (uFSH) concentrations were determined in 14 regularly menstruating volunteers aged 23-39 years during one complete menstrual cycle. (More)
The gold standard of semen analysis is still an manual method, which is time-consuming, labour intensive and needs thorough quality control. Microfluidics can also offer advantages for this application. Therefore a first step in the development of a microfluidic chip has been made, which enables the man the semen analysis at home. In this article recent(More)
OBJECTIVE To determine whether women who do not conceive during an IVF treatment despite normal FSH levels have a higher rate of apoptosis in their granulosa-lutein cells than women who do conceive. DESIGN Prospective. SETTING Large teaching hospital and fertility center. PATIENT(S) Patients with normal FSH levels undergoing an IVF treatment. (More)
After the introduction of assays determining apoptosis in human ejaculated spermatozoa, several studies have been published about the relationship between apoptosis in spermatozoa and semen quality. Apoptosis in spermatozoa is significantly correlated with conventional semen quality parameters, but also with the outcome of assisted reproductive techniques.(More)
The gold standard of semen analysis is still an manual method, which is time‐consuming, labour intensive and needs thorough quality control. Microfluidics can also offer advantages for this application. Therefore a first step in the development of a microfluidic chip has been made, which enables the man the semen analysis at home. In this article recent(More)