G Hannequart

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Washed bacterial suspensions obtained from the pig hindgut were incubated under 13CO2 in a buffer containing NaH13CO3 and carbohydrates. Incorporation of 13C into short chain fatty acids was assayed by quantitative nuclear magnetic resonance. The effects of different levels of H2 added to the gas phase (0, 20 and 80% v/v) and of the specific methanogenesis(More)
The rumen simulation technique (Rusitec) has been used to study the effects of phosphorus (P) deficiency on bacterial protein synthesis and chemical composition and on adenosine triphosphate (ATP) concentrations in the solid and liquid phases of fermentors. 16 g DM of a P-deficient mixed diet was put into each vessel daily and the vessels were infused with(More)
Nuclear magnetic resonance (NMR) was used to study the metabolism of the rumen bacterial ecosystem. The fermentation of 1-13C-glucose was investigated. This system showed a transient build-up and decay of lactate labelled at the C3 position. The degradation of lactate gave rise to volatile fatty acids. These results agree with in vivo data on diets rich in(More)
The fermentation of [1-13C] glucose and [3-13C]lactate by bacteria isolated from sheep rumen and pig large intestine was compared by the nuclear magnetic resonance (NMR) technique. Washed cell suspensions were incubated directly in the NMR spectrometer and spectra were recorded every 10 min after injection of the labelled substrates. The results showed(More)
Four semi-purified diets with different calcium (Ca) and phosphorus (P) contents were given to adult sheep at a level of 1 kg daily plus 100 g of straw. The phosphorus was supplied as dicalcium phosphate, almost insoluble in rumen fluid. In the preliminary period, all the animals (16) were fed the test diet (NCa-NP) containing 8.4 g of Ca and 3.3. g of P.(More)
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