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Numerous human embryonic stem cell lines with different genetic background are widely used as cell models for fundamental, biomedical and pharmacological research. New hES cell lines SC5, SC6, SC7, and SC3a are derived from the blastocysts and maintained on mitotically inactivated human feeder cells. All derived hES cell lines passed through more than 120(More)
The Karyotypic structure of some clones and subclones of Chinese hamster cells has been studied (CHO-KI). 60-80% of all the clonal cells demonstrated the identical karyotype (the main structural variant of karyotype - MSVK). The distribution of cells according to the number of chromosomes does not vary within 90 passages of clone cultivation (about 300 cell(More)
The karyotypic structure of the Indian muntjak skin fibroblast cell line, with 7 chromosomes in modal class, was compared in the cells cultures, with 10 and 3% of fetal calf serum of two different lots. In addition, the effect of the same serum lot on chromosome variability in the two different Indian muntjac skin fibroblast sublines differing in the modal(More)
The numerical karyotypic variability has been investigated in "markerless" epithelial-like Rat kangaroo kidney cell lines NBL-3-11 and NBL-3-17 on cultivation on a laminin-2/4 coated surface. In cell line NBL-3-17, cultivated on the laminin-coated surface for 2, 4 and 12 days, the character of numerical karyotypic variability has changed. In 2 days the(More)
The numerical and structural karyotypic variability has been investigated in the Indian muntjac skin fibroblasts cell line M and karyotypic variant of this line M' on cultivation on a laminin 2/4 coated surface. In cell line M, cultivated on the laminin-coated surface for 4 and 14 days, and in karyotypic variant M', cultivated for 2, 4 and 14 days, the(More)
New nonimmortalized fibroblast-like cell lines SC5-MSC and SC3a-MSC, FetMSC, FRSN were obtained from human embryonic stem cells (ESC), bone marrow of a 5-6-days embryo and foreskin of a 3-years-old boy, respectively. All the lines are successfully used as the feeder at human ESC cultivation. It is determined that the average cell population doublings time(More)
The karyotypic variability has been investigated for an immortalized human epithelioid cervix carcinoma cell line M HeLa clone 11, cultivated for 15-60 days after contamination with Acholeplasma laidlawii A, strain PG-8, and for 30 days after contamination with Mycoplasma arginini R-16. The character of cell distribution for chromosome number changes in(More)
The structural karyotypic variability has been investigated in the "markerless" epithelial-like Rat kangaroo kidney cell lines NBL-3-17 and NBL-3-11 on cultivation on a laminin-2/4 coated surface. In cell line NBL-3-17, cultivated on the laminin-coated surface for 2, 4 and 12 days, and in cell line NBL-3-11, cultivated on the laminin-coated surface for 2(More)
A study was made of the karyotypic structure of sublines derived from the kangaroo rat's kidney (NBL-3) and skin fibroblasts of the Indian muntjac, available in the cell culture bank of the Institute of Cytology Acad. Sci. USSR. A comparative karyologic analysis was made of subline NBL-3 both contaminated with mycoplasma (NBLK) and decontaminated with(More)
Four continuous human embryonic stem cell lines (SC1, SC2, SC3 and SC4), derived from the blastocysts has been described. The cell lines were cultivated on mitotically inactivated human feeder cells. The cell lines SC1 and SC2 have passed through 150 population doublings and the cell lines SC3 and SC4 -- near 120 populations doublings, which exceeds(More)