G G Polianskaia

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The Karyotypic structure of some clones and subclones of Chinese hamster cells has been studied (CHO-KI). 60-80% of all the clonal cells demonstrated the identical karyotype (the main structural variant of karyotype - MSVK). The distribution of cells according to the number of chromosomes does not vary within 90 passages of clone cultivation (about 300 cell(More)
A long-term cultivation (5-8 months) of human blastocyst-derived embryonic cells (hES) was performed. Several properties of hESs were examined to prove the state of continuous cell lines. These cells have passed through 100-175 population doublings with the average population doubling time equal to 37.0 +/- 1.5 h. Isolated hESs, referred to as HESC-1,(More)
Karyotypic variability has been studied in a line of the Chinese hamster cells artificially contaminated with Mycoplasma arginini. The contaminated cultures differed from mycoplasma-free cells in cell distribution for chromosome number. The frequency of cells with modal chromosome number 21 decreased, while that of cells with 20 chromosomes increased.(More)
A new feeder-free culture system for human embryonic stem cells (hESC) was developed. It consist of extracellular matrix proteins synthesized by feeder cells--mesenchymal stem cell line SC5-MSC, which was derived from initial hESC line SC5. The major ECM proteins--fibronectin and laminin--that maintain hESC growth in feeder-free system were identified. An(More)
Hybrids between non-malignant mouse 3T3 cells and highly malignant rat JF1 cells were selected in semisolid agar medium without asparagine: 3T3 cells do not grow in agar; JF1 cells required asparagine for their growth. Some surface properties: agglutination by Con A, saturation density, electrophoretic mobility and behaviour in the two-phase aqueous polymer(More)
The karyotypic variability has been investigated for an immortalized human epithelioid cervix carcinoma cell line M HeLa clone 11, cultivated for 15-60 days after contamination with Acholeplasma laidlawii A, strain PG-8, and for 30 days after contamination with Mycoplasma arginini R-16. The character of cell distribution for chromosome number changes in(More)
The karyotypic structure of the Indian muntjak skin fibroblast cell line, with 7 chromosomes in modal class, was compared in the cells cultures, with 10 and 3% of fetal calf serum of two different lots. In addition, the effect of the same serum lot on chromosome variability in the two different Indian muntjac skin fibroblast sublines differing in the modal(More)
A study was made of the karyotypic structure of sublines derived from the kangaroo rat's kidney (NBL-3) and skin fibroblasts of the Indian muntjac, available in the cell culture bank of the Institute of Cytology Acad. Sci. USSR. A comparative karyologic analysis was made of subline NBL-3 both contaminated with mycoplasma (NBLK) and decontaminated with(More)
The structural karyotypic variability has been investigated in the "markerless" epithelial-like Rat kangaroo kidney cell lines NBL-3-17 and NBL-3-11 on cultivation on a laminin-2/4 coated surface. In cell line NBL-3-17, cultivated on the laminin-coated surface for 2, 4 and 12 days, and in cell line NBL-3-11, cultivated on the laminin-coated surface for 2(More)
The numerical and structural karyotypic variability has been investigated in the Indian muntjac skin fibroblasts cell line M and karyotypic variant of this line M' on cultivation on a laminin 2/4 coated surface. In cell line M, cultivated on the laminin-coated surface for 4 and 14 days, and in karyotypic variant M', cultivated for 2, 4 and 14 days, the(More)