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Cadmium is a widespread industrial pollutant. The primary route of exposure occurs via contaminated drinking water or food supplies, and tobacco. Its chronic introduction and ingestion lead to bio-magnification in target organs, as the liver. The aim of this paper is to determine Cd cytotoxic concentrations in the human hepatoma cell line HepG2. Further(More)
The accurate spectral characterization of high-resolution spectrometers is required for correctly computing, interpreting, and comparing radiance and reflectance spectra acquired at different times or by different instruments. In this paper, we describe an algorithm for the spectral characterization of field spectrometer data using sharp atmospheric or(More)
Two-dimensional light-scattering patterns from aggregates have undergone feature extraction followed by multivariate statistical analysis. The aggregates are comprised of primary particles of varying shape and size. Morphological descriptors (features) were extracted by a nonlinear filtering algorithm (spectrum enhancement) and then processed by principal(More)
Carcinogenesis is a multi-step process involving genetic alterations and non-genotoxic mechanisms. The in vitro cell transformation assay allows the monitoring of the neoplastic phenotype by foci formation in suitable cells (e.g. C3H10T1/2 mouse embryo fibroblasts) showing aberrant morphology of massive build-up, polar and multi-layered densely stained(More)
A classifier capable of ranking structural alterations of the cytoskeleton is developed. Images of cytoskeletal microtubules obtained from the epifluorescence microscopy of primary culture rat hepatocytes are analyzed. Morphological descriptors are extracted by contour and mass fractal analysis, direct methods, and spectrum enhancement. All methods are(More)
Inverse problems aimed at locating cracks and voids inside a dielectric medium by means of electromagnetic waves involve knowledge of transmission eigenvalues. The subject has been actively investigated by many Authors for some years and results have been presented in articles and books. Unfortunately, the frequency dependence of dielectric permittivity and(More)
The cytoskeleton is a network of proteins which structurally and dynamically organise the cytoplasm of living cells. Microtubules are among its constituents. Morphological alterations of microtubules are related to functional impairment. Therefore cytoskeletal morphology is a valuable indicator of cell injury and functionality. This paper focuses on the(More)
OBJECTIVE In vitro models of hematopoiesis used in investigative hematopathology and in safety studies on candidate drugs, involve clonogenic assays on colony-forming unit granulocyte macrophage (CFU-GM). These assays require live and unstained colonies to be counted. Most laboratories still rely on visual scoring, which is time-consuming and error-prone.(More)