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An Agrobacterium-mediated transformation system with glyphosate selection has been developed for the large-scale production of transgenic plants. The system uses 4-day precultured immature embryos as explants. A total of 30 vectors containing the 5-enol-pyruvylshikimate-3-phosphate synthase gene from Agrobacterium strain CP4 (aroA:CP4), which confers(More)
Large scale production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] by Aeromonas hydrophila 4AK4 was examined in a 20,000 l fermentor. Cells were first grown using glucose as a carbon source, and polyhydroxyalkanoate (PHA) biosynthesis was triggered by the addition of lauric acid under conditions of limited nitrogen or phosphorus. When(More)
During two epidemics of acute diarrhoea in China in late 1982/early 1983, more than 12 000 adults in two coal mining districts were affected. The virus isolated from stool samples resembled a rotavirus morphologically. Antigenically it lacked the group antigen shared by known rotaviruses. Like other rotaviruses it had a double-stranded RNA with 11 discrete(More)
Continental-scale estimations of terrestrial methane (CH4) and nitrous oxide (N2O) fluxes over a long time period are crucial to accurately assess the global balance of greenhouse gases and enhance our understanding and prediction of global climate change and terrestrial ecosystem feedbacks. Using a process-based global biogeochemical model, the Dynamic(More)
Chronically elevated blood glucose (hyperglycaemia) is the primary indicator of type 2 diabetes, which has a prevalence that varies considerably by ethnicity in the USA, with African-Americans disproportionately affected. Genome-wide association studies (GWASs) have significantly enhanced our understanding of the genetic basis of diabetes and related(More)
A simultaneous measurement of the top-quark, W-boson, and neutrino masses is reported for tt events selected in the dilepton final state from a data sample corresponding to an integrated luminosity of 5.0 fb−1 collected by the CMS experiment in pp collisions at √ s = 7 TeV. The analysis is based on endpoint determinations in kinematic distributions. When(More)
Gene segment 9 of the adult diarrheal rotavirus, ADRV, has been cloned, and the full-length gene 9 cDNA has been sequenced. Sequences at the 5' and 3' termini of the gene 9 double-stranded RNA were confirmed by direct RNA sequencing. The ninth RNA segment is 814 bases in length with a single open reading frame beginning at base 10 and terminating at base(More)
Organ and tissue loss or failure resulting from an injury or other type of damage is a major human health problem. Tissue or organ transplantation is a standard therapy to treat these patients, but this is severely limited by donor shortage. Other available therapies including surgical reconstruction, drug therapy, synthetic prostheses, and medical devices(More)
The records of 34 patients diagnosed with primary small bowel non-Hodgkin's lymphoma during a 10-year period between January 1996 and December 2005, including 27 cases for which complete follow-up records were available, were studied. Abdominal pain (70.6% of patients) was the main presenting symptom, followed by intestinal obstruction (38.2%). The most(More)
Evidence is presented for the associated production of a single top quark and W boson in pp collisions at sqrt[s]=7  TeV with the CMS experiment at the LHC. The analyzed data correspond to an integrated luminosity of 4.9  fb(-1). The measurement is performed using events with two leptons and a jet originated from a b quark. A multivariate analysis based on(More)