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Emerging next-generation sequencing technologies have revolutionized the collection of genomic data for applications in bioforensics, biosurveillance, and for use in clinical settings. However, to make the most of these new data, new methodology needs to be developed that can accommodate large volumes of genetic data in a computationally efficient manner.(More)
Excess postexercise oxygen consumption (EPOC) may describe the impact of previous exercise on energy metabolism. Ten males completed Resistance Only, Run Only, Resistance-Run, and Run-Resistance experimental conditions. Resistance exercise consisted of 7 lifts. Running consisted of 25 minutes of treadmill exercise. Vo(2) was determined during treadmill(More)
Twelve lactating, multiparous Holstein cows were used to evaluate the effects of silage chop length and proportion of long hay in low and adequate forage fiber diets on chewing activities and productivity of cows fed barley-based concentrates. Diets were designed with two forage:concentrate ratios (low forage fiber, 35:65; adequate forage fiber, 65:35),(More)
Twelve lactating, multiparous Holstein cows were used in two experiments, designed as 6 x 6 Latin squares with 28-d periods, to evaluate the effects of increasing the NDF concentration of diets consisting of barley-based concentrates and various forages. Diets were designed in a 3 x 2 factorial set with three concentrations of total dietary NDF (32, 36, and(More)
Spectral analysis techniques were used to characterize the cyclical variation in rumination behavior of cows. Four Holstein cows were fed twice daily a diet of 60% high-moisture shelled corn-based concentrate, 15% first-cut alfalfa-grass hay and 25% second-cut alfalfa silage. The number of minutes that each cow spent ruminating was determined for 15-min(More)
Medical implants are sometimes colonized by biofilm-forming bacteria, which are very difficult to treat effectively. The combination of gentamicin and ultrasonic exposure for 24 hours was previously shown to reduce the viability of Escherichia coli biofilms in vivo. This article shows that such treatment for 48 hours reduced viable E coli bacteria to nearly(More)
Escherichia coli biofilms on two polyethylene disks were implanted subcutaneously into rabbits receiving systemic gentamicin. Ultrasound was applied for 24 h to one disk. Both disks were removed, and viable bacteria were counted. Pulsed ultrasound significantly reduced bacterial viability below that of nontreated biofilms without damage to the skin.
OBJECTIVE To evaluate the validity, reliability, sensitivity, and specificity of the PaciTemp supralingual digital pacifier thermometer as compared to the Thermoscan Instant tympanic and glass-mercury rectal thermometers. METHOD Eighty-one children under the age of 2 years had temperatures taken sequentially at three body sites: supralingual, tympanic,(More)
The hypothesis that ultrasound increases antibiotic transport through biofilms of Escherichia coli and Pseudomonas aeruginosa was investigated using colony biofilms. Biofilms grown on membrane filters were transferred to nutrient agar containing 50 microg/ml gentamicin. A smaller filter was placed on top of the biofilm and a blank concentration disk was(More)
Infection of implanted medical devices by Gram-positive organisms such as Staphylococcus ssp. is a serious concern in the biomaterial community. In this research the application of low frequency ultrasound to enhance the activity of vancomycin against implanted Staphylococcus epidermidis biofilms was examined. Polyethylene disks covered with a biofilm of S.(More)